| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
The biological target of N-6-Methyl-2-deoxyadenosine-d3 is not a receptor or enzyme in the traditional sense, as it is an isotopically labeled analog used as an analytical standard. The parent compound, N-6-Methyl-2-deoxyadenosine, is a DNA nucleoside modification involved in epigenetic regulation.
|
|---|---|
| ln Vitro |
In vitro, N-6-Methyl-2-deoxyadenosine-d3 does not exert pharmacological activity; it is used as an internal standard in analytical chemistry. Its "activity" is its performance in mass spectrometry, where it serves as a reference for the accurate quantification of the non-deuterated form.
|
| ln Vivo |
In vivo activity is not applicable to N-6-Methyl-2-deoxyadenosine-d3, as it is an analytical standard and not a pharmacologically active compound. It is not used in animal studies for therapeutic purposes.
|
| Enzyme Assay |
In vitro enzyme or receptor binding assay protocols are not applicable to N-6-Methyl-2-deoxyadenosine-d3. Instead, it is used in analytical protocols, particularly in liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. A standard protocol involves spiking a known amount of the deuterated internal standard into a biological sample, followed by extraction, chromatographic separation, and mass spectrometric detection.
|
| Cell Assay |
In vitro cell-based assay protocols are not applicable to N-6-Methyl-2-deoxyadenosine-d3, as it is not used in cell-based studies for its biological effects. Its use is limited to analytical chemistry applications.
|
| Animal Protocol |
In vivo animal experimental protocols are not applicable to N-6-Methyl-2-deoxyadenosine-d3, as it is an analytical standard and not a pharmacologically active compound for in vivo studies.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to N-6-Methyl-2-deoxyadenosine-d3 as a drug. It is a stable isotope-labeled compound with a molecular weight of 268.29. It is soluble in DMSO. Its stability and storage conditions are well-defined for use as a reference material.
|
| Toxicity/Toxicokinetics |
Toxicological properties are not applicable to N-6-Methyl-2-deoxyadenosine-d3 as a drug. It is an analytical standard and is not intended for human use. Standard laboratory safety precautions should be followed when handling this chemical.
|
| References | |
| Additional Infomation |
N-6-Methyl-2-deoxyadenosine-d3 is a research-grade stable isotope-labeled compound used as an internal standard for the quantification of N-6-methyl-2-deoxyadenosine (m⁶dA), a DNA epigenetic modification. It has no therapeutic applications and has not entered clinical trials. The compound is available exclusively for research purposes.
|
| Molecular Formula |
C11N5O3H15
|
|---|---|
| Molecular Weight |
268.287
|
| Exact Mass |
268.136
|
| CAS # |
1354782-02-7
|
| PubChem CID |
169437423
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
192-198°C
|
| LogP |
0
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
19
|
| Complexity |
321
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
[2H]C([2H])([2H])NC1=C2C(=NC=N1)N(C=N2)[C@H]3C[C@@H]([C@H](O3)CO)O
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7273 mL | 18.6366 mL | 37.2731 mL | |
| 5 mM | 0.7455 mL | 3.7273 mL | 7.4546 mL | |
| 10 mM | 0.3727 mL | 1.8637 mL | 3.7273 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
