| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
N6-Methyladenine does not have a specific receptor or enzyme target in the traditional sense, as it is a modified DNA base rather than a pharmacologically active compound. However, it is a substrate for various DNA methyltransferases and demethylases that regulate DNA methylation. In prokaryotes, N6-Methyladenine is involved in restriction-modification systems, where it serves as a marker for self-DNA and protects against foreign DNA. In eukaryotes, N6-methyladenine has been studied as an epigenetic mark, and enzymes that add or remove this modification are potential targets for research. The compound is also used as a reference standard for studying DNA methylation and epigenetic regulation. |
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| ln Vitro |
In vitro, N6-Methyladenine is used as a research tool to study DNA methylation and epigenetic regulation. It can be used as a standard in analytical methods for the detection and quantification of methylated DNA bases. The compound is also used in studies of DNA methyltransferases and demethylases to understand their substrate specificity and catalytic mechanisms. As a modified nucleobase, N6-Methyladenine can be incorporated into synthetic DNA oligonucleotides to study the effects of DNA methylation on protein-DNA interactions, transcription, and DNA repair. However, specific pharmacological activity data such as IC50 values have not been reported, as the compound is not used as a drug but as a research tool.
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| ln Vivo |
In vivo activity is not applicable to N6-Methyladenine, as the compound is a modified DNA base and analytical standard rather than a pharmacologically active compound. It is not used in animal studies for therapeutic purposes. Instead, it is used as a reference standard for the quantification of methylated DNA bases in biological samples. Endogenous N6-methyladenine plays important roles in prokaryotic DNA metabolism, but the compound itself is not administered to animals for its biological effects.
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| Enzyme Assay |
In vitro enzyme or receptor binding assay protocols are not applicable to N6-Methyladenine, as it is a modified DNA base and analytical standard rather than a pharmacologically active compound. Instead, it is used in analytical protocols, particularly in liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the quantification of methylated DNA bases. A standard protocol involves hydrolyzing DNA samples to release nucleobases, followed by chromatographic separation and mass spectrometric detection. N6-Methyladenine is used as a reference standard for identification and quantification. The compound can also be used in biochemical assays to study DNA methyltransferase activity, where it serves as a substrate or product for the enzyme reaction.
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| Cell Assay |
In vitro cell-based assay protocols are not applicable to N6-Methyladenine, as it is a modified DNA base and analytical standard and is not used in cell-based studies for its biological effects. Its use is limited to analytical chemistry and epigenetic research applications.
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| Animal Protocol |
In vivo animal experimental protocols are not applicable to N6-Methyladenine, as it is an analytical standard and not a pharmacologically active compound for in vivo studies.
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| References | |
| Additional Infomation |
6-Methyladenine is a methyladenine, a product of 9H-purine-6-amine with a methyl group replacing the amino nitrogen atom. It is a human metabolite. It is a 6-alkylaminopurine, and also a methyladenine.
6-Methyladenine has been reported to be detected in Trypanosoma brevicornu, and relevant data are available for reference. N6-Methyladenine (CAS#: 443-72-1) is a research-grade modified purine base that is widely present in prokaryotes. It plays important roles in DNA replication, transcription, mismatch repair, and chromosome replication in prokaryotes. In epigenetics and genetic research, it offers insights into the mechanisms of DNA methylation and gene regulation. The compound has a molecular weight of 149.15 and a molecular formula of C6H7N5. It has no therapeutic applications and has not entered clinical trials. The compound is available for research purposes. |
| Molecular Formula |
C6H7N5
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|---|---|
| Molecular Weight |
149.15
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| Exact Mass |
149.07
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| CAS # |
443-72-1
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| PubChem CID |
67955
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| Appearance |
Solid powder
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| Density |
1.476g/cm3
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| Boiling Point |
492.9ºC at 760mmHg
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| Melting Point |
≥300ºC
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| Flash Point |
251.9ºC
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| Vapour Pressure |
0.0153mmHg at 25°C
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| Index of Refraction |
1.792
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| LogP |
0.467
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
11
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| Complexity |
139
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CNC1=C2NC=NC2=NC=N1
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| InChi Key |
CKOMXBHMKXXTNW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H7N5/c1-7-5-4-6(10-2-8-4)11-3-9-5/h2-3H,1H3,(H2,7,8,9,10,11)
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| Chemical Name |
N-methyl-7H-purin-6-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL | |
| 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL | |
| 10 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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