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| Other Sizes |
| Targets |
Sphingosine-1-phosphate (d17:1) targets the same biological pathways as endogenous sphingosine-1-phosphate (S1P), a bioactive lipid that acts as a signaling molecule. S1P binds to a family of five G protein-coupled receptors (S1P1-5) that regulate various cellular processes, including cell survival, proliferation, migration, and angiogenesis. S1P is produced by the phosphorylation of sphingosine by sphingosine kinases (SphK1 and SphK2) and is degraded by S1P lyase. Sphingosine-1-phosphate (d17:1) is a synthetic analog that contains a 17-carbon sphingoid base, making it structurally similar to the endogenous S1P but distinguishable by mass spectrometry. As an internal standard, it is used to accurately quantify endogenous S1P levels in biological samples.
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| ln Vitro |
In vitro, Sphingosine-1-phosphate (d17:1) is not typically evaluated for pharmacological activity, as it is primarily used as an analytical standard. Its primary in vitro application is in mass spectrometry-based lipidomics for the quantification of endogenous S1P levels. The compound serves as an internal standard to correct for losses during sample preparation and to ensure accurate quantification. Endogenous S1P, which the d17:1 analog mimics, is a bioactive lipid that contributes to cell growth and function and is involved in various cellular processes. However, the d17:1 analog itself is not used to study these effects, as it is a synthetic standard rather than a biologically active compound in its own right.
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| ln Vivo |
In vivo activity is not applicable to Sphingosine-1-phosphate (d17:1), as the compound is an analytical standard rather than a pharmacologically active compound. It is not used in animal studies for therapeutic purposes. Instead, it is used to quantify endogenous S1P levels in biological samples collected from animal models of disease. Endogenous S1P plays important roles in various physiological and pathological processes, including atherosclerosis, inflammation, immunity, and tumorigenesis. However, Sphingosine-1-phosphate (d17:1) itself is not administered to animals for its biological effects.
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| Enzyme Assay |
In vitro enzyme or receptor binding assay protocols are not applicable to Sphingosine-1-phosphate (d17:1), as it is an analytical standard rather than a pharmacologically active compound. Instead, it is used in analytical protocols, particularly in liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the quantification of S1P. A standard protocol involves adding a known amount of the d17:1 internal standard to a biological sample (e.g., plasma, tissue homogenate, or cell lysate), followed by lipid extraction, chromatographic separation, and mass spectrometric detection. The ratio of endogenous S1P to the internal standard is used to calculate the absolute concentration of S1P in the sample.
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| Cell Assay |
In vitro cell-based assay protocols are not applicable to Sphingosine-1-phosphate (d17:1), as it is an analytical standard and not used in cell-based studies for its biological effects. Its use is limited to analytical chemistry applications for the quantification of S1P.
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| Animal Protocol |
In vivo animal experimental protocols are not applicable to Sphingosine-1-phosphate (d17:1), as it is an analytical standard and not a pharmacologically active compound for in vivo studies.
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| References |
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| Additional Infomation |
C17 sphingosine-1-phosphate is a phosphoric acid ethanolamine.
Sphingosine-1-phosphate (d17:1) is an analytical standard used for the quantification of sphingosine-1-phosphate (d18:1) by mass spectrometry. It has a molecular weight of 365.45 and a molecular formula of C17H36NO5P. The compound is a derivative of ceramide and can be used for cancer research. It has no therapeutic applications and has not entered clinical trials. Its mechanism of action is not applicable, as it is used as an internal standard rather than as a biologically active compound. The compound is available for research purposes. |
| Molecular Formula |
C17H36NO5P
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|---|---|
| Molecular Weight |
365.45
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| Exact Mass |
365.233
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| CAS # |
474923-27-8
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| PubChem CID |
5283559
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| Appearance |
White to off-white solid powder
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| LogP |
4.351
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
16
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| Heavy Atom Count |
24
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| Complexity |
359
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CCCCCCCCCCCC/C=C/[C@H]([C@H](COP(=O)(O)O)N)O
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| InChi Key |
VITGNIYTXHNNNE-LHMZYYNSSA-N
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| InChi Code |
InChI=1S/C17H36NO5P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-17(19)16(18)15-23-24(20,21)22/h13-14,16-17,19H,2-12,15,18H2,1H3,(H2,20,21,22)/b14-13+/t16-,17+/m0/s1
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| Chemical Name |
[(E,2S,3R)-2-amino-3-hydroxyheptadec-4-enyl] dihydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7364 mL | 13.6818 mL | 27.3635 mL | |
| 5 mM | 0.5473 mL | 2.7364 mL | 5.4727 mL | |
| 10 mM | 0.2736 mL | 1.3682 mL | 2.7364 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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