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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
AF 594 azide does not have a specific biological target. Its function is as a fluorescent labeling reagent for biomolecules. The azide group reacts with alkyne-functionalized molecules via copper-catalyzed or copper-free click chemistry (SPAAC). The AF 594 fluorophore provides bright and photostable red fluorescence for imaging and detection applications. The compound is used to label proteins, nucleic acids, and other biomolecules that have been modified with alkyne groups.
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| ln Vitro |
In vitro, AF 594 azide is used as a fluorescent labeling reagent for detecting and visualizing alkyne-labeled biomolecules. The compound's high fluorescence quantum yield and photostability make it suitable for various imaging applications. The azide-alkyne click reaction allows for specific and efficient labeling of target molecules. The compound's spectral properties (excitation at 594 nm, emission at 613-615 nm) are compatible with standard red fluorescence detection channels.
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| ln Vivo |
In vivo, AF 594 azide is not typically used as it is a fluorescent labeling reagent for in vitro applications. The compound may be used in ex vivo imaging of labeled tissues or cells. Its use in vivo would require appropriate formulation and consideration of its pharmacokinetic properties. The compound is primarily used in research applications for labeling and detecting biomolecules in cell culture and tissue sections.
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| Enzyme Assay |
In vitro assays for AF 594 azide involve click chemistry reactions with alkyne-containing molecules. The compound is incubated with alkyne-labeled biomolecules under click reaction conditions (copper-catalyzed or copper-free). Labeling efficiency is assessed by measuring fluorescence intensity or by gel electrophoresis followed by fluorescence scanning. The compound's photostability is evaluated by continuous illumination and monitoring of fluorescence intensity over time.
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| Cell Assay |
In vitro cellular assays for AF 594 azide involve labeling alkyne-modified biomolecules within cells. Cells are treated with alkyne-labeled precursors (e.g., alkyne-modified amino acids, nucleotides, or sugars) that are incorporated into cellular components. AF 594 azide is then applied to the cells, and the click reaction labels the alkyne-modified biomolecules. Labeled cells are visualized by fluorescence microscopy or analyzed by flow cytometry. Cell viability is assessed to ensure that the labeling procedure does not cause cytotoxicity.
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| Animal Protocol |
In vivo animal studies for AF 594 azide are not typically performed, as the compound is a fluorescent labeling reagent rather than a therapeutic or diagnostic agent. If used in vivo, the compound would be injected and its biodistribution and clearance evaluated. However, such studies are not standard for this compound. The compound is primarily used for labeling cells, tissues, or biomolecules ex vivo or in vitro.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for AF 594 azide are not applicable, as the compound is a fluorescent labeling reagent and not a drug. The compound is typically used in vitro or ex vivo and is not administered systemically for therapeutic purposes. If used in vivo for imaging, its pharmacokinetic properties would depend on the formulation and the specific application. However, such data are not reported in the available literature.
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| Toxicity/Toxicokinetics |
Toxicological data for AF 594 azide are limited. As a fluorescent labeling reagent, the compound is intended for research use only and is not intended for human consumption or therapeutic use. The compound's azide group may be potentially reactive, but at the low concentrations used for labeling, toxicity is minimal. Standard safety precautions should be taken when handling the compound. Comprehensive toxicological assessments have not been reported.
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| Additional Infomation |
AF 594 azide triethylamine is a fluorescent labeling reagent used in research applications. Its azide group enables click chemistry labeling of alkyne-functionalized biomolecules. The compound exhibits high fluorescence quantum yield and photostability, with excitation at 594 nm and emission at 613-615 nm. It is used for labeling proteins, nucleic acids, and other biomolecules for imaging and detection. The compound is not approved for clinical use and is intended for research purposes only.
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| Molecular Formula |
C44H55N7O10S2
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|---|---|
| Molecular Weight |
906.08
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| Appearance |
Pale purple to purple solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1037 mL | 5.5183 mL | 11.0366 mL | |
| 5 mM | 0.2207 mL | 1.1037 mL | 2.2073 mL | |
| 10 mM | 0.1104 mL | 0.5518 mL | 1.1037 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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