| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
PEGs
Not applicable. This is a linker molecule without a specific biological target. Its "target" is the functional groups it can react with (e.g., azides for click chemistry, amines/thiols for substitution). |
|---|---|
| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
Not applicable. As a chemical linker, it has no biological activity of its own. Its utility is in forming stable triazole linkages via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry, or in nucleophilic substitution reactions via the bromide . |
| ln Vivo |
No in vivo activity. The compound is purely a chemical reagent for conjugation and is not intended for direct biological testing.
|
| Enzyme Assay |
Not applicable. This is a chemical linker used in test tubes for synthesis, not for biological binding assays.
|
| Cell Assay |
Not applicable. The compound is not used in cell culture experiments. Its biological effects can only be assessed after it is conjugated to active drug moieties.
|
| Animal Protocol |
Not applicable. This reagent is not administered to animals.
|
| ADME/Pharmacokinetics |
Molecular weight is 207.07 g/mol. It has a purity of 97%. As a PEG-based linker, it improves the water solubility and flexibility of conjugates it is incorporated into .
|
| Toxicity/Toxicokinetics |
No toxicity data available. Standard laboratory safety precautions should be taken when handling this alkylating reagent due to the presence of the reactive bromide group.
|
| References | |
| Additional Infomation |
This compound is a versatile building block for chemical biology. It allows the introduction of a clickable alkyne handle into a molecule, or the creation of a linker for conjugating two entities via the bromide. It is a research-grade reagent for synthesis, not a drug candidate.
|
| Molecular Formula |
C7H11BRO2
|
|---|---|
| Molecular Weight |
207.065041780472
|
| Exact Mass |
205.994
|
| CAS # |
1287660-82-5
|
| PubChem CID |
53253864
|
| Appearance |
Light yellow to yellow liquid
|
| LogP |
0.8
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
10
|
| Complexity |
105
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
BrCCOCCOCC#C
|
| InChi Key |
SEVJGPXPOQIEPS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H11BrO2/c1-2-4-9-6-7-10-5-3-8/h1H,3-7H2
|
| Chemical Name |
3-[2-(2-bromoethoxy)ethoxy]prop-1-yne
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8293 mL | 24.1464 mL | 48.2928 mL | |
| 5 mM | 0.9659 mL | 4.8293 mL | 9.6586 mL | |
| 10 mM | 0.4829 mL | 2.4146 mL | 4.8293 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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