| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
PEGs
PROTAC Linkers; Biotin-binding proteins (avidin/streptavidin). |
|---|---|
| ln Vitro |
PROTAC linkers serve the purpose of joining the two distinct ligands that comprise PROTACs; these ligands are, respectively, the target protein and E3 ubiquitin ligase[1]. Target proteins are specifically degraded by PROTACs by taking advantage of the intracellular ubiquitin-proteasome system[1]. can considerably slow the growth of tumors in tumor xenografts[1]. When coupled with protein G (compound 6), biotin-PEG3-C3-NH2 (compound 11) (0-2 mM; 3 h) exhibits fluorescence under streptavidin probes. Biotin-PEG3-C3-NH2 is an effective tool for high-throughput analysis of protein-small molecule interaction and profiling enzyme activities in microarrays made using the surface derivatization method[3].
As a linker molecule, Biotin-PEG3-C3-NH2 itself has no intrinsic biological activity; it serves as a structural connector to join a target protein ligand and an E3 ubiquitin ligase ligand in PROTAC synthesis. The biotin group enables the capture and detection of the final PROTAC or its protein targets using streptavidin-based assays. |
| ln Vivo |
No specific in vivo activity has been reported for this linker alone; its activity is derived from the final PROTAC molecule after conjugation with appropriate ligands. In vivo, the biotinylated PROTAC could be used to study target engagement and tissue distribution using avidin-based probes.
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| Enzyme Assay |
N/A; this compound is not assessed in enzyme/receptor binding assays. Its quality is confirmed by analytical methods such as HPLC and NMR, with a standard purity of ≥98% and a molecular weight of 446.6. The biotin-streptavidin interaction can be confirmed by a colorimetric or fluorescence-based binding assay.
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| Cell Assay |
N/A; this linker is not tested alone in cell-based assays. In a typical protocol, it is conjugated to a target ligand and an E3 ligase ligand to form a biotinylated PROTAC. Cells are then treated with the PROTAC, lysed, and the biotinylated species are captured on streptavidin-coated beads. Bound proteins are eluted and identified by Western blotting or mass spectrometry.
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| Animal Protocol |
N/A; no animal studies are performed with the linker alone. For a complete biotinylated PROTAC, in vivo studies could involve administering the compound to animals, then harvesting tissues, preparing lysates, and performing streptavidin pull-downs to assess target engagement and PROTAC distribution.
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| ADME/Pharmacokinetics |
This compound has a molecular weight of 446.6, a molecular formula of C20H38N4O5S, and is typically stored as a powder at -20degC for up to 3 years, sealed and away from moisture, or in a solvent at -80degC for 6 months or at -20degC for 1 month. It is soluble in methanol (≥175 mg/mL).
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| Toxicity/Toxicokinetics |
This product is for research use only and is not for human therapeutic or clinical applications. Standard chemical safety precautions should be observed during handling. The amine group is reactive and should be stored away from acids and oxidizing agents.
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| References |
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| Additional Infomation |
Biotin-PEG3-C3-NH2 is an effective tool for high-throughput analysis of protein-small molecule interactions and profiling enzyme activities in microarrays constructed by surface derivatization. The PEG3 spacer improves biotin accessibility, ensuring strong and consistent binding to streptavidin probes. This linker enables the direct detection and affinity capture of PROTACs and their cellular targets.
|
| Molecular Formula |
C20H38N4O5S
|
|---|---|
| Molecular Weight |
446.60
|
| Exact Mass |
446.256
|
| CAS # |
183896-00-6
|
| Related CAS # |
rel-Biotin-PEG3-C3-NH2;1374658-86-2
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| PubChem CID |
12068534
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| Appearance |
Colorless to light yellow ointment
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| Density |
1.139±0.06 g/cm3(Predicted)
|
| Boiling Point |
715.3±60.0 °C(Predicted)
|
| LogP |
-0.6
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
18
|
| Heavy Atom Count |
30
|
| Complexity |
500
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCOCCOCCOCCCN)NC(=O)N2
|
| InChi Key |
XNGTVIOREZIKAE-LNLFQRSKSA-N
|
| InChi Code |
InChI=1S/C20H38N4O5S/c21-7-3-9-27-11-13-29-14-12-28-10-4-8-22-18(25)6-2-1-5-17-19-16(15-30-17)23-20(26)24-19/h16-17,19H,1-15,21H2,(H,22,25)(H2,23,24,26)/t16-,17-,19-/m0/s1
|
| Chemical Name |
5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[3-[2-[2-(3-aminopropoxy)ethoxy]ethoxy]propyl]pentanamide
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2391 mL | 11.1957 mL | 22.3914 mL | |
| 5 mM | 0.4478 mL | 2.2391 mL | 4.4783 mL | |
| 10 mM | 0.2239 mL | 1.1196 mL | 2.2391 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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