| Size | Price | Stock | Qty |
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| 5mg |
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| Targets |
PEGs
Azide-PEG3-Desthiobiotin does not have a specific biological target itself but serves as a bioconjugation and affinity purification reagent. The desthiobiotin moiety binds reversibly to streptavidin, allowing for gentle elution of biotinylated molecules from streptavidin affinity columns. The azide group enables bioorthogonal click chemistry conjugation to alkyne-functionalized molecules. The PEG3 spacer provides water solubility and reduces steric hindrance during conjugation. |
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| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
In vitro studies demonstrate that Azide-PEG3-Desthiobiotin functions as a bioconjugation and affinity purification reagent. The azide group allows for efficient conjugation to alkyne-containing molecules via CuAAc click reactions. The desthiobiotin moiety enables reversible binding to streptavidin, allowing for gentle elution under mild conditions. The PEG3 spacer provides water solubility and minimizes steric hindrance during conjugation. In vitro applications include labeling of biomolecules, affinity purification, and detection of alkyne-modified molecules. |
| ln Vivo |
In vivo activity data for Azide-PEG3-Desthiobiotin as a standalone compound are not reported, as it is utilized as a bioconjugation and affinity purification reagent rather than as a therapeutic agent. The compound is designed for in vitro applications in protein labeling, affinity purification, and detection. Its in vivo applications would depend on the specific conjugated molecule and the overall properties of the complete bioconjugate.
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| Enzyme Assay |
In vitro assays for Azide-PEG3-Desthiobiotin typically involve click conjugation studies to assess its reactivity with alkyne-containing molecules. The azide group can be conjugated to alkyne-functionalized molecules via CuAAc click reactions, and conjugation efficiency can be assessed by techniques such as mass spectrometry, HPLC, or gel electrophoresis. Streptavidin binding assays can be performed to evaluate the reversible binding of the desthiobiotin moiety to streptavidin. Elution efficiency can be assessed under various conditions.
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| Cell Assay |
In vitro cell-based assays using Azide-PEG3-Desthiobiotin typically involve labeling of cellular components or biomolecules. Cells or cell lysates containing alkyne-modified biomolecules can be treated with Azide-PEG3-Desthiobiotin via click chemistry for labeling and detection. The desthiobiotin moiety allows for subsequent affinity purification or detection using streptavidin-conjugated probes. Labeling efficiency and specificity can be assessed by Western blotting, fluorescence microscopy, or mass spectrometry.
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| Animal Protocol |
In vivo animal studies using Azide-PEG3-Desthiobiotin are not typically performed, as the compound is primarily used as an in vitro bioconjugation and affinity purification reagent. The compound is intended for research use in labeling and purification applications.
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| ADME/Pharmacokinetics |
As a bioconjugation reagent rather than a therapeutic drug, comprehensive pharmacokinetic data for Azide-PEG3-Desthiobiotin alone are not available. The compound has a molecular formula of C18H34N6O5 and a molecular weight of 414.51. The compound typically appears as a liquid. It has a purity of ≥95%. It should be stored at -20°C under inert gas. The desthiobiotin moiety enables reversible streptavidin binding for affinity purification applications.
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| Toxicity/Toxicokinetics |
The toxicity profile of Azide-PEG3-Desthiobiotin as an individual compound is not extensively characterized, as it is primarily used as a research reagent. The compound is intended for research use only and is not approved for therapeutic use in humans. Azide-containing compounds can be potentially explosive and should be handled with appropriate safety precautions. Standard laboratory safety practices, including the use of personal protective equipment and handling in a fume hood, are recommended.
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| References | |
| Additional Infomation |
Azide-PEG3-Desthiobiotin (CAS 1426828-06-9) has a molecular formula of C18H34N6O5 and a molecular weight of 414.51. It is a versatile bioconjugation reagent designed for reversible affinity purification and labeling applications. The compound contains an azide group for click chemistry and a desthiobiotin moiety for reversible streptavidin binding. It has a purity of ≥95% and should be stored at -20°C under inert gas.
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| Molecular Formula |
C18H34N6O5
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|---|---|
| Molecular Weight |
414.499763965607
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| Exact Mass |
414.259
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| CAS # |
1426828-06-9
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| PubChem CID |
86580437
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| Appearance |
Light yellow to brown liquid
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| LogP |
1
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
29
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| Complexity |
515
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C([C@H]1NC(=O)N[C@H]1C)CCCCC(=O)NCCOCCOCCOCCN=[N+]=[N-]
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| InChi Key |
JIQVKRRASYKCEL-CVEARBPZSA-N
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| InChi Code |
InChI=1S/C18H34N6O5/c1-15-16(23-18(26)22-15)5-3-2-4-6-17(25)20-7-9-27-11-13-29-14-12-28-10-8-21-24-19/h15-16H,2-14H2,1H3,(H,20,25)(H2,22,23,26)/t15-,16+/m1/s1
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| Chemical Name |
N-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl]-6-[(4S,5R)-5-methyl-2-oxoimidazolidin-4-yl]hexanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4125 mL | 12.0627 mL | 24.1255 mL | |
| 5 mM | 0.4825 mL | 2.4125 mL | 4.8251 mL | |
| 10 mM | 0.2413 mL | 1.2063 mL | 2.4125 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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