| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
L-Tryptophan-d5 does not have a specific biological target itself, as it is an analytical standard. However, its unlabeled counterpart, L-tryptophan, is an essential amino acid that serves as a precursor for the biosynthesis of serotonin, melatonin, and niacin. It is involved in protein synthesis and various metabolic pathways. As an internal standard, L-Tryptophan-d5 is used to accurately quantify L-tryptophan levels in biological samples, which is important for studying its role in various physiological and pathological processes.
|
|---|---|
| ln Vitro |
L-Tryptophan-d5 is not typically used for in vitro activity studies in the same way as pharmacologically active compounds. However, it is used in in vitro assays as an internal standard for the investigation of anti-inflammatory activity of low molecular weight fractions. It is also used in structural elucidation of micronutrients and nutraceutical components present in foodstuffs using LC-MS/MS. In biophysical studies, it is used for neutron scattering experiments.
|
| ln Vivo |
As an analytical internal standard, L-Tryptophan-d5 is not used for in vivo activity studies. However, the unlabeled L-tryptophan has been extensively studied in vivo for its role as an essential amino acid and precursor for various bioactive molecules. The labeled compound is used as a reference standard in bioanalytical methods to accurately quantify L-tryptophan levels in biological samples from in vivo studies, such as plasma, urine, or tissue homogenates.
|
| Enzyme Assay |
As an analytical internal standard, L-Tryptophan-d5 is not typically used in enzyme/receptor binding assays. However, it may be used as an internal standard in assays that measure the binding of L-tryptophan or its metabolites to receptors or enzymes. In such assays, the labeled compound is added to samples to correct for variability in sample preparation and instrument performance, ensuring accurate quantification of the unlabeled analyte.
|
| Cell Assay |
L-Tryptophan-d5 is not used in cell-based assays as a bioactive compound. However, it may be used as an internal standard in cell-based assays that measure the uptake, metabolism, or release of L-tryptophan from cultured cells. In these applications, the labeled compound is added to cell culture media or cell lysates to enable accurate quantification of L-tryptophan levels by LC-MS/MS.
|
| Animal Protocol |
In vivo animal studies using L-Tryptophan-d5 are not typically performed, as the compound is an analytical internal standard. However, the compound may be administered to animals in metabolic tracing studies to track the fate of tryptophan in vivo. In such studies, the labeled compound is used to distinguish between endogenous and exogenous tryptophan and to study its metabolism and distribution in various tissues.
|
| ADME/Pharmacokinetics |
As an analytical standard, L-Tryptophan-d5 has a molecular formula of C11H7D5N2O2 (indole-d5) and a molecular weight of 209.25. It has an isotopic purity of 97 atom % D. The compound is typically supplied as a powder and should be stored according to the manufacturer's recommendations. As a stable isotope-labeled compound, it has near-identical physicochemical properties to unlabeled L-tryptophan, making it an ideal internal standard for quantitative bioanalysis.
|
| Toxicity/Toxicokinetics |
The toxicity profile of L-Tryptophan-d5 as an individual compound is not extensively characterized, as it is used as an analytical standard in very small quantities. The unlabeled L-tryptophan is an essential amino acid that is generally recognized as safe at normal dietary levels. However, the labeled compound is intended for research use only and is not approved for therapeutic use in humans. Standard laboratory safety practices should be followed when handling this compound.
|
| References | |
| Additional Infomation |
L-Tryptophan-d5 (Tryptophan-d5; Tryptophane-d5) (CAS 62595-11-3) is a stable isotope-labeled internal standard (SIL-IS) intended for use as an internal standard for the quantification of L-tryptophan by GC- or LC-MS. L-Tryptophan is an essential amino acid. The compound is also known as L-Tryptophan-(indole-d5). It is recognized as the gold standard for quantitative bioanalysis due to its near-identical physicochemical properties to the unlabeled analyte.
|
| Molecular Formula |
C11H7D5N2O2
|
|---|---|
| Molecular Weight |
209.26
|
| Exact Mass |
209.121
|
| CAS # |
62595-11-3
|
| Related CAS # |
L-Tryptophan;73-22-3
|
| PubChem CID |
12209747
|
| Appearance |
White to off-white solid powder
|
| Density |
1.396 g/cm3
|
| Boiling Point |
447.9ºC at 760 mmHg
|
| Melting Point |
280-285ºC (dec.)(lit.)
|
| Flash Point |
224.7ºC
|
| LogP |
1.822
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
15
|
| Complexity |
245
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
[2H]C1=C(C(=C2C(=C1[2H])C(=C(N2)[2H])C[C@@H](C(=O)O)N)[2H])[2H]
|
| InChi Key |
QIVBCDIJIAJPQS-HLTLGYGQSA-N
|
| InChi Code |
InChI=1S/C11H12N2O2/c12-9(11(14)15)5-7-6-13-10-4-2-1-3-8(7)10/h1-4,6,9,13H,5,12H2,(H,14,15)/t9-/m0/s1/i1D,2D,3D,4D,6D
|
| Chemical Name |
(2S)-2-amino-3-(2,4,5,6,7-pentadeuterio-1H-indol-3-yl)propanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO :≥ 33.33 mg/mL (~159.28 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7787 mL | 23.8937 mL | 47.7874 mL | |
| 5 mM | 0.9557 mL | 4.7787 mL | 9.5575 mL | |
| 10 mM | 0.4779 mL | 2.3894 mL | 4.7787 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
