| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
DL-Alanine is an amino acid used as a reducing and capping agent in nanoparticle synthesis. D-Alanine is a component of bacterial cell walls, while L-alanine is involved in sugar and acid metabolism.
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|---|---|
| ln Vitro |
In vitro, DL-Alanine-13C-1 is used as a tracer to study alanine metabolism in cell cultures. It is also used as a reducing and capping agent for nanoparticle generation with aqueous silver nitrate solution.
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| ln Vivo |
In vivo, this compound is administered to animals as a tracer to study whole-body alanine kinetics and metabolism.
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| Enzyme Assay |
DL-Alanine-13C-1 is used as an internal standard for LC-MS/MS quantification of alanine in biological samples. It is also extensively used in NMR spectroscopy to investigate molecular structures and dynamics.
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| Cell Assay |
For cell studies, DL-Alanine-13C-1 is added to culture medium (e.g., RPMI, DMEM) to study alanine metabolism in hepatocytes and neurons.
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| Animal Protocol |
In animal studies, DL-Alanine-13C-1 is typically administered to rodents via intravenous tracer infusion to study whole-body alanine kinetics and metabolism.
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| ADME/Pharmacokinetics |
DL-Alanine-13C-1 has identical ADME properties to unlabeled alanine. It is rapidly absorbed, metabolized in the liver to pyruvate, and has a short plasma half-life.
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| Toxicity/Toxicokinetics |
DL-Alanine is generally recognized as safe (GRAS). The labeled analog is considered non-toxic for research use.
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| References | |
| Additional Infomation |
DL-Alanine-13C-1 is used as a research tool for metabolic flux analysis, NMR spectroscopy, and as an internal standard for LC-MS/MS quantification of alanine in biological samples.
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| Molecular Formula |
C213CH7NO2
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|---|---|
| Molecular Weight |
90.09
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| Exact Mass |
89.047
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| CAS # |
102029-81-2
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| Related CAS # |
DL-Alanine;302-72-7
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| PubChem CID |
3086470
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
212.9±23.0 °C at 760 mmHg
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| Melting Point |
289ºC (dec.)(lit.)
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| Flash Point |
82.6±22.6 °C
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| Vapour Pressure |
0.1±0.9 mmHg at 25°C
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| Index of Refraction |
1.460
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| LogP |
-0.68
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
6
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| Complexity |
61.8
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC([13C](=O)O)N
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| InChi Key |
QNAYBMKLOCPYGJ-LBPDFUHNSA-N
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| InChi Code |
InChI=1S/C3H7NO2/c1-2(4)3(5)6/h2H,4H2,1H3,(H,5,6)/i3+1
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| Chemical Name |
2-amino(113C)propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 11.1000 mL | 55.5001 mL | 111.0001 mL | |
| 5 mM | 2.2200 mL | 11.1000 mL | 22.2000 mL | |
| 10 mM | 1.1100 mL | 5.5500 mL | 11.1000 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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