| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
DL-5-Hydroxylysine is a hydroxylated form of lysine, integral to the formation of stable cross-links in collagen fibers, enhancing the structural integrity of connective tissues.
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|---|---|
| ln Vitro |
In vitro, DL-5-Hydroxylysine hydrochloride is used as a potential target for free radical-induced protein oxidation. It is used in biochemical assays to study collagen biosynthesis and stabilization.
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| ln Vivo |
In vivo, this compound is used to study collagen metabolism and connective tissue disorders. It is involved in lysine degradation pathways and contributes to the understanding of amino acid metabolism.
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| Enzyme Assay |
A non-cell assay uses collagen or synthetic peptides containing hydroxylysine. The compound can be used as a standard in HPLC or LC-MS to analyze hydroxylysine content in hydrolyzed tissue samples as a marker for collagen turnover.
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| Cell Assay |
For cell studies, DL-5-Hydroxylysine hydrochloride is dissolved in culture medium and added to fibroblast or osteoblast cultures to study collagen synthesis and cross-linking. Hydroxyproline and hydroxylysine levels in cell lysates are measured by HPLC.
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| Animal Protocol |
In animal studies, DL-5-Hydroxylysine hydrochloride is typically administered to rodents to study lysine degradation pathways and collagen metabolism. It is used in models of connective tissue disorders and bone metabolism.
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| ADME/Pharmacokinetics |
The compound is absorbed from the gut and distributed to connective tissues, where it is incorporated into collagen. It is metabolized via lysine degradation pathways and is renally cleared. Its half-life is short (hours).
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| Toxicity/Toxicokinetics |
DL-5-Hydroxylysine is non-toxic at physiological concentrations as it is a normal constituent of collagen. The hydrochloride salt may cause mild gastrointestinal irritation at high doses.
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| Additional Infomation |
DL-5-Hydroxylysine hydrochloride is used as a standard for the analysis of hydroxylysine in clinical diagnostics and research, particularly in studies of collagen metabolism and connective tissue disorders. It is also used as a potential target marker for radical-induced protein oxidation in proteomics research.
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| Molecular Formula |
C6H15CLN2O3
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|---|---|
| Molecular Weight |
198.65
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| Exact Mass |
198.077
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| CAS # |
13204-98-3
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| PubChem CID |
2724399
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| Appearance |
White to off-white solid powder
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| Melting Point |
225 °C (dec.)(lit.)
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| LogP |
0.7
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
12
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| Complexity |
129
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CC(C(=O)O)N)C(CN)O.Cl
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| InChi Key |
MJXVOTKVFFAZQJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H14N2O3.ClH/c7-3-4(9)1-2-5(8)6(10)11;/h4-5,9H,1-3,7-8H2,(H,10,11);1H
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| Chemical Name |
2,6-diamino-5-hydroxyhexanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :≥ 100 mg/mL (~503.40 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0340 mL | 25.1699 mL | 50.3398 mL | |
| 5 mM | 1.0068 mL | 5.0340 mL | 10.0680 mL | |
| 10 mM | 0.5034 mL | 2.5170 mL | 5.0340 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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