| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 25mg | |||
| Other Sizes |
| Targets |
SAM is endogenously produced from methionine and ATP and acts as a universal methyl donor for transmethylation reactions. It is a substrate for DNA methyltransferases (DNMTs), protein methyltransferases (PRMTs), and other methyltransferases.
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|---|---|
| ln Vitro |
In vitro, SAM-d3 is used as a tracer to study methyl group transfer and metabolic pathways in biochemical and analytical research. Unlabeled SAM has anti-proliferative, pro-apoptotic, and anti-metastatic effects in cancer research.
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| ln Vivo |
In vivo, SAM is a dietary supplement with potent antidepressant effects and liver-protective properties. The labeled version is used as a tracer to study methylation reactions and SAM metabolism in animal models.
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| Enzyme Assay |
A non-cell assay uses purified methyltransferase (e.g., DNMT) and a DNA or protein substrate. SAM-d3 is added as the methyl donor, and deuterium-labeled methylated products are quantified by LC-MS to measure enzyme activity.
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| Cell Assay |
For cell studies, SAM-d3 is added to culture medium (typically 10-100 uM) and incubated with cells (e.g., hepatocytes, cancer cell lines). Incorporation of deuterated methyl groups into DNA, RNA, and proteins is analyzed by LC-MS or isotopic ratio mass spectrometry.
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| Animal Protocol |
In animal studies, SAM-d3 is typically administered to rodents by intraperitoneal injection or oral gavage (50-200 mg/kg). Blood and tissue samples (liver, brain) are collected to analyze SAM levels and methylation status of DNA and histones by LC-MS.
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| ADME/Pharmacokinetics |
SAM is rapidly absorbed and distributed to all tissues, particularly the liver and brain. It has a short plasma half-life (1-2 hours). It is metabolized to S-adenosyl-L-homocysteine (SAH), which is further metabolized to homocysteine.
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| Toxicity/Toxicokinetics |
SAM is generally well-tolerated at therapeutic doses. Mild side effects may include gastrointestinal discomfort. The labeled analog is considered safe for research use.
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| References |
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| Additional Infomation |
S-Adenosyl-L-methionine-d3 is used as a research tool for studying methylation reactions, epigenetics, and metabolic flux analysis. Unlabeled SAM is an approved dietary supplement for the treatment of depression, osteoarthritis, and liver disease in many countries.
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| Molecular Formula |
C15H19D3N6O5S
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|---|---|
| Molecular Weight |
401.46
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| Exact Mass |
401.156
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| CAS # |
68684-40-2
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| Related CAS # |
S-Adenosyl-L-methionine;29908-03-0;S-Adenosyl-L-methionine (1,4-butanedisulfonate);200393-05-1
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| PubChem CID |
162641963
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| Appearance |
White to off-white solid powder
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| LogP |
-2.8
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
27
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| Complexity |
527
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O[C@@H]1[C@@H]([C@@H](C[S+](C([2H])([2H])[2H])CC[C@H](N)C(=O)[O-])O[C@H]1N1C=NC2=C(N=CN=C12)N)O
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| InChi Key |
MEFKEPWMEQBLKI-JSJAHMFWSA-N
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| InChi Code |
InChI=1S/C15H22N6O5S/c1-27(3-2-7(16)15(24)25)4-8-10(22)11(23)14(26-8)21-6-20-9-12(17)18-5-19-13(9)21/h5-8,10-11,14,22-23H,2-4,16H2,1H3,(H2-,17,18,19,24,25)/t7-,8+,10+,11+,14+,27?/m0/s1/i1D3
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| Chemical Name |
(2S)-2-amino-4-[[(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl-(trideuteriomethyl)sulfonio]butanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~50 mg/mL (~124.55 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4909 mL | 12.4545 mL | 24.9091 mL | |
| 5 mM | 0.4982 mL | 2.4909 mL | 4.9818 mL | |
| 10 mM | 0.2491 mL | 1.2455 mL | 2.4909 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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