| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Pyridoxine-d3 hydrochloride targets pyridoxine metabolic pathways; it is converted to pyridoxal 5'-phosphate (PLP), the active coenzyme form of vitamin B6; it serves as a tracer for vitamin B6 metabolism and as an internal standard for LC-MS/MS quantification.
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|---|---|
| ln Vitro |
In vitro, Pyridoxine-d3 hydrochloride is used as an internal standard in cell culture and biochemical assays for quantifying pyridoxine and its metabolites; it is metabolized by cells in the same manner as unlabeled pyridoxine, enabling accurate measurement of vitamin B6 levels.
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| ln Vivo |
In vivo, Pyridoxine-d3 hydrochloride is administered to animals or humans as a tracer to study vitamin B6 metabolism; it is absorbed, phosphorylated to pyridoxal 5'-phosphate, and excreted as metabolites; the deuterium label allows precise tracking of pyridoxine disposition.
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| Enzyme Assay |
Non-cellular assay procedure: Pyridoxine-d3 hydrochloride is dissolved in aqueous solution and spiked into biological samples (e.g., plasma, urine, or tissue homogenates); samples are extracted and analyzed by LC-MS/MS; the deuterated compound serves as an internal standard for isotope dilution quantification of pyridoxine and its metabolites.
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| Cell Assay |
Cellular assays: Cells are cultured with Pyridoxine-d3 hydrochloride in the medium; at various time points, cells and media are harvested; metabolites are extracted and analyzed by LC-MS/MS to measure pyridoxine uptake, phosphorylation, and conversion to pyridoxal 5'-phosphate; isotopologue distribution is used to study metabolic flux.
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| Animal Protocol |
In vivo animal experiments: Pyridoxine-d3 hydrochloride is administered to rodents via oral gavage or intravenous injection; blood, urine, and tissue samples are collected over time; pyridoxine and its metabolites are quantified by LC-MS/MS using the deuterated compound as an internal standard to study pharmacokinetics and tissue distribution.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties: Pyridoxine-d3 hydrochloride behaves identically to unlabeled pyridoxine; it is well absorbed orally, phosphorylated in the liver to pyridoxal 5'-phosphate, and excreted in urine as 4-pyridoxic acid; the deuterium label does not significantly affect its pharmacokinetic profile.
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| Toxicity/Toxicokinetics |
Toxicological profile: Pyridoxine-d3 hydrochloride is a stable isotope-labeled vitamin with low toxicity; vitamin B6 is safe at nutritional doses; high doses may cause neuropathy; as a research reagent, standard laboratory safety precautions apply.
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| References | |
| Additional Infomation |
Other information: Pyridoxine-d3 hydrochloride is used as an internal standard for vitamin B6 analysis in clinical, nutritional, and pharmaceutical research; the deuterium label at the methyl group provides a +3 Da mass shift for mass spectrometry detection.
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| Molecular Formula |
C8H9D3CLNO3
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|---|---|
| Molecular Weight |
208.66
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| Exact Mass |
208.069
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| CAS # |
1189921-12-7
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| Related CAS # |
Pyridoxine hydrochloride;58-56-0
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| PubChem CID |
46782823
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
13
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| Complexity |
142
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])C1=NC=C(C(=C1O)CO)CO.Cl
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| InChi Key |
ZUFQODAHGAHPFQ-NIIDSAIPSA-N
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| InChi Code |
InChI=1S/C8H11NO3.ClH/c1-5-8(12)7(4-11)6(3-10)2-9-5;/h2,10-12H,3-4H2,1H3;1H/i1D3;
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| Chemical Name |
4,5-bis(hydroxymethyl)-2-(trideuteriomethyl)pyridin-3-ol;hydrochloride
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| Synonyms |
Pyridoxol-d3 (hydrochloride); Vitamin B6-d3 (hydrochloride)
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7925 mL | 23.9624 mL | 47.9249 mL | |
| 5 mM | 0.9585 mL | 4.7925 mL | 9.5850 mL | |
| 10 mM | 0.4792 mL | 2.3962 mL | 4.7925 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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