| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
L-Methionine-13C, like L-methionine, is an essential amino acid that serves as a precursor for protein synthesis and as a methyl donor in various metabolic pathways. It targets metabolic enzymes involved in amino acid metabolism and transmethylation reactions.
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|---|---|
| ln Vitro |
In vitro, L-Methionine-13C is used as a tracer to study methionine metabolism and protein synthesis in cell culture. The ¹³C label allows for the tracking of methionine-derived carbon atoms into proteins and other metabolites.
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| ln Vivo |
In vivo, L-Methionine-13C is used as a tracer to study methionine metabolism in animals and humans. It can be administered orally or intravenously, and the ¹³C label is tracked in blood, tissues, and exhaled breath to study metabolic flux.
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| Enzyme Assay |
Non-cellular assays for L-Methionine-13C typically involve its use as an internal standard in analytical chemistry. It is spiked into samples, and the ratio of labeled to unlabeled methionine is measured by mass spectrometry to quantify the amount of methionine present.
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| Cell Assay |
In vitro cellular assays for L-Methionine-13C involve culturing cells with the labeled amino acid. The incorporation of ¹³C into proteins and other metabolites can be measured by mass spectrometry to study protein synthesis and metabolic pathways.
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| Animal Protocol |
In vivo animal experiments for L-Methionine-13C involve administering the labeled amino acid to animals. Blood, tissue, and breath samples are collected over time, and the ¹³C enrichment in methionine and its metabolites is measured to study methionine kinetics and metabolism.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) properties: L-Methionine-13C behaves identically to unlabeled L-methionine. It is absorbed from the gastrointestinal tract, distributed to tissues, and metabolized via transmethylation and transsulfuration pathways. The ¹³C label does not alter its pharmacokinetic profile.
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| Toxicity/Toxicokinetics |
Toxicological profile: L-Methionine-13C is a stable isotope-labeled essential amino acid with low toxicity. L-methionine is safe at nutritional doses. High doses may have adverse effects, but the labeled compound is used at tracer levels.
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| References | |
| Additional Infomation |
L-Methionine-13C has a molecular weight of 150.20 and a molecular formula of ¹³CC₄H₁₁NO₂S. Its CAS number is 49705-26-2. It is supplied for research purposes as a metabolic tracer and analytical standard.
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| Molecular Formula |
C413CH11NO2S
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|---|---|
| Molecular Weight |
150.20
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| Exact Mass |
149.051
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| CAS # |
49705-26-2
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| Related CAS # |
L-Methionine;63-68-3
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| PubChem CID |
15556502
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
306.9±37.0 °C at 760 mmHg
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| Melting Point |
284ºC(lit.)
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| Flash Point |
139.4±26.5 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.531
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| LogP |
0.37
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
9
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| Complexity |
97
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[13CH3]SCC[C@@H](C(=O)O)N
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| InChi Key |
FFEARJCKVFRZRR-YWQIHCTDSA-N
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| InChi Code |
InChI=1S/C5H11NO2S/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1/i1+1
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| Chemical Name |
(2S)-2-amino-4-(113C)methylsulfanylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6578 mL | 33.2889 mL | 66.5779 mL | |
| 5 mM | 1.3316 mL | 6.6578 mL | 13.3156 mL | |
| 10 mM | 0.6658 mL | 3.3289 mL | 6.6578 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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