| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Guidelines (This is our suggested protocol; it should be adjusted to meet your unique needs; it is merely a guideline). 1. Dimethyl sulfoxide (DMSO) is used to dissolve MQA-P to create a stock solution (1.0 mM). 2. for ONOO-imaging in live cells. HeLa cells were subjected to three different treatments: 30 minutes of pretreatment with SIN-1 (HY-126849; 100 μM) followed by 30 minutes of incubation with MQA-P (5 μM) as a control. Green channel: λem = 550–670 nm, λex = 405 nm. 3. Imaging of live cell viscosity. HeLa cells were subjected to three different treatments: a 30-minute pretreatment with monensin (10 μM) followed by a 30-minute incubation with MQA-P (5 μM) as a control. Using a confocal laser scanning microscope with red channel (λex=561 nm, λem=680-750 nm), fluorescence pictures were captured. 4. Dual-channel imaging of ferroptosis polarity, viscosity, and ONOO-. HeLa cells were treated with MQA-P (5 μM) for 30 minutes, followed by a 30-minute pretreatment with Erastin (HY-15763; 50 μM) and another 30-minute incubation with MQA-P (5 μM). Using confocal laser scanning microscopy, fluorescence images were obtained for viscosity and polarity in the red channel (λex=561 nm, λem=680-750 nm) and ONOO-in the green channel (λex=405 nm, λem=550-670 nm) [1].
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|---|---|
| ln Vivo |
Guidelines (This is our suggested protocol; it should be adjusted based on your unique requirements as it just offers as a guideline). 1. Isolate normal organs (heart, liver, spleen, lungs, and kidney) and cancers, then cut each into pieces that are 5 μm thick for tissue section imaging. 2. After incubating the sections for 30 minutes with MQA-P (20 μM), they were rinsed three times with PBS (pH 7.4) and photographed in vivo using a confocal laser scanning microscope. For ONOO-, the green channel (λex=405 nm, λem=550-670 nm) was used, and for viscosity and polarity, the red channel (λex=561 nm, λem=680-750 nm) was used [1].
|
| References |
| Molecular Formula |
C40H36BRN2O2P
|
|---|---|
| Molecular Weight |
687.60
|
| Appearance |
Typically exists as solid at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4543 mL | 7.2717 mL | 14.5433 mL | |
| 5 mM | 0.2909 mL | 1.4543 mL | 2.9087 mL | |
| 10 mM | 0.1454 mL | 0.7272 mL | 1.4543 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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