| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
As an internal standard, the compound does not have a biological target. However, the non-labeled parent compound, 1-Methylinosine, is a modified nucleotide found at position 37 in tRNA (3' to the anticodon) of eukaryotic tRNA. It is a biomarker for RNA turnover and is found at higher concentrations in the urine of patients with certain cancers, such as breast cancer.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantification throughout the drug development process. Due to its potential to alter the pharmacokinetic and metabolic characteristics of medications, deuteration has drawn attention[1].
The compound itself has no biological activity; it is a stable isotope-labeled internal standard. The non-labeled 1-Methylinosine is a naturally occurring modified nucleoside. Its levels in urine are studied as a biomarker for cancer diagnosis. The d3-labeled standard mimics the behavior of the natural compound in analytical systems. |
| Enzyme Assay |
A standard LC-MS/MS protocol is used. A known amount of the d3-labeled internal standard is spiked into biological samples (e.g., plasma, urine). After protein precipitation and solid-phase extraction, the sample is injected onto a C18 column. The analyte and internal standard are detected in MRM mode, and the peak area ratio is used for precise quantification.
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| Cell Assay |
The compound is not used in cell viability assays. Instead, it is added as an internal standard to cellular extracts. For instance, cancer cells can be cultured, and the media can be collected. The d3-internal standard is spiked into the media before LC-MS/MS analysis to quantify the amount of 1-Methylinosine secreted by the cells.
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| Animal Protocol |
The internal standard is not administered in animal studies. In a pharmacokinetic or biomarker study, urine or blood samples are collected from animals. The d3-internal standard is spiked into these samples to accurately measure the concentration of the endogenous nucleoside 1-Methylinosine. This helps study metabolic flux or validate biomarker changes.
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| ADME/Pharmacokinetics |
1-Methylinosine-d3 has a molecular weight of 285.27 and is a solid white to off-white powder. It is soluble in DMSO and water. It is stable when stored at -20degC in a sealed container away from moisture and light. As an analytical standard, it co-elutes with the non-labeled analyte in LC.
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| Toxicity/Toxicokinetics |
The labeled compound is non-toxic at the trace concentrations used for analytical purposes. The parent compound, 1-Methylinosine, is an endogenous modified nucleoside that is naturally present in the body. The product is "For research use only" and not for human therapeutic or diagnostic use.
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| References | |
| Additional Infomation |
1-Methylinosine-d3 is a research-grade stable isotope-labeled chemical (Internal Standard) used for LC-MS/MS quantification. It is not a drug and has no FDA approval for therapeutic use. It is used in pharmaceutical research for biomarker quantitation, particularly for studying RNA modification and cancer metabolism. The non-labeled compound is a known urinary biomarker for breast cancer.
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| Molecular Formula |
C11H11D3N4O5
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|---|---|
| Molecular Weight |
285.27
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| Related CAS # |
1-Methylinosine;2140-73-0
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5055 mL | 17.5273 mL | 35.0545 mL | |
| 5 mM | 0.7011 mL | 3.5055 mL | 7.0109 mL | |
| 10 mM | 0.3505 mL | 1.7527 mL | 3.5055 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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