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| 5mg |
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| 10mg |
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| Targets |
The unlabeled parent compound, 4',7-Dimethoxyisoflavone, targets fungal cells and exhibits antifungal properties. It is a flavonoid compound classified as an isoflavone. The compound is studied for its potential biological activities, including anti-inflammatory, antioxidant, and anticancer effects, though it is largely used for research related to drug development and metabolic studies.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an impact on a drug's pharmacokinetics and metabolic profile, it has drawn attention [1].
The non-labeled 4',7-Dimethoxyisoflavone has been shown in vitro to have antifungal activity against certain plant pathogenic fungi. It is a flavonoid compound that may exert its effects through multiple cellular pathways, but its specific mechanism of action is not fully elucidated. The deuterated form is not used for activity assays. |
| ln Vivo |
The in vivo activity of the parent compound is not well-documented. It is a naturally occurring flavonoid from plant sources. The compound's primary application is in metabolic studies and analytical method development. The deuterated version is strictly a research tool for quantitation and is not used for in vivo efficacy studies.
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| Enzyme Assay |
To assess binding affinity or enzyme inhibition, standard cell-free assays for isoflavones can be adapted, such as using radioligand binding or enzyme inhibition assays, though this compound is used as an internal standard. The compound can be used as a tracer for quantitation during the drug development process. One common method is LC-MS analysis.
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| Cell Assay |
The compound is utilized as an internal standard for LC-MS/MS analysis. For example, it can be added to cell homogenates to measure the concentration of the parent compound or other isoflavones in cellular metabolism studies. The d6 internal standard is added to all standards and samples at the beginning of the extraction process to ensure analytical accuracy.
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| Animal Protocol |
This compound is not administered to animals as a therapeutic drug. Its use in animal studies is indirect; it is spiked into collected plasma or tissue samples as an internal standard to analyze the concentration of other drugs or endogenous metabolites. This helps validate the pharmacokinetic profiles of other compounds.
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| ADME/Pharmacokinetics |
It is a stable isotope-labeled chemical with high purity (>99%). It has good stability and can be stored as a powder at -20degC. As an analytical standard, its key property is its ability to co-elute with the non-labeled analyte while being distinguishable by mass spectrometry. This allows for highly precise quantification.
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| Toxicity/Toxicokinetics |
As a non-toxic, stable isotope-labeled flavonoid, it is safe for analytical use. It is not intended for human or animal consumption. The unlabeled parent compound, 4',7-Dimethoxyisoflavone, has low toxicity and is generally recognized as safe for research purposes at standard concentrations.
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| References | |
| Additional Infomation |
This product is a research-grade stable isotope-labeled chemical (Internal Standard) for advanced pharmaceutical and biochemical research. It is used for analytical method development, method validation (AMV), and Quality Controlled (QC) applications. It is not a drug and has no FDA approval for therapeutic use. It is strictly for research applications.
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| Molecular Formula |
C17H8D6O4
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| Molecular Weight |
288.33
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| Related CAS # |
4',7-Dimethoxyisoflavone;1157-39-7
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4682 mL | 17.3412 mL | 34.6825 mL | |
| 5 mM | 0.6936 mL | 3.4682 mL | 6.9365 mL | |
| 10 mM | 0.3468 mL | 1.7341 mL | 3.4682 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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