| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
The target is the serotonin (5-HT) metabolic pathway, where 5-hydroxy tryptophol beta-D-glucuronide serves as a phase II metabolite of tryptophan-derived serotonin. As a tracer, the deuterated compound binds and behaves identically to the unlabeled analyte. The deuterium label allows precise tracking of the metabolite in complex biological matrices.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantification throughout the drug development process. Due to its potential to alter the pharmacokinetic and metabolic characteristics of medications, deuteration has drawn attention[1].
The compound itself has no direct biological activity. The parent, non-labeled 5-hydroxy tryptophol beta-D-glucuronide, participates in the serotonin metabolic pathway. The stable isotope labeling is crucial for drug development, acting largely as a tracer for quantitation during the drug development process, and the d4 label enhances analytical accuracy. |
| ln Vivo |
No inherent in vivo activity is documented for the labeled compound. The non-labeled metabolite is involved in normal tryptophan metabolism via the serotonin pathway. The labeled compound is a research tool for in vivo and in vitro studies to accurately measure the amount of the metabolite in biological samples using mass spectrometry-based assays.
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| Enzyme Assay |
A standard LC-MS/MS assay protocol is used: a known amount of the deuterated internal standard is added to biological samples (e.g., plasma, urine, or tissue homogenates). After protein precipitation, centrifugation, and C18 column separation, the analyte and internal standard are detected in multiple reaction monitoring (MRM) mode. The peak area ratio is used for precise quantification.
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| Cell Assay |
The compound is not used in cell viability assays. Instead, it is added as an internal standard to cellular lysates or supernatants. For example, to study serotonin metabolism in neuronal cells, cells are cultured and the media is collected. The d4-internal standard is added before LC-MS/MS analysis. This method allows for the simultaneous measurement of multiple analytes in a single run.
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| Animal Protocol |
The compound is not administered directly to animals in efficacy studies. It is used in pharmacokinetic studies for method validation. For example, after administration of a drug that affects serotonin metabolism, blood or urine samples are collected from rats. The d4-internal standard is spiked into these samples before LC-MS/MS analysis to accurately measure the concentration of the endogenous metabolite.
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| ADME/Pharmacokinetics |
The deuterated compound has high stability and solubility. The Molecular Formula is C16H15D4NO8 and Molecular Weight is 357.35. It is essential for advanced pharmaceutical and biochemical research, offering precise and reliable analytical results. It integrates seamlessly into existing protocols and is suitable for high-precision scientific investigations.
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| Toxicity/Toxicokinetics |
The labeled compound is non-toxic at the trace concentrations used for analytical purposes. The parent metabolites are endogenous substances produced during normal metabolic processes. Comprehensive toxicity studies are not required for an internal standard. The product is labeled "for research use only" and is not intended for human or animal therapeutic use.
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| References | |
| Additional Infomation |
This product is a research-grade stable isotope-labeled compound (Internal Standard) used for LC-MS/MS quantification in advanced pharmaceutical and biochemical research. It is crucial for neurochemistry studies involving serotonin metabolism and pharmacokinetics. It is not a drug candidate and has no FDA approval. It is strictly for research use only.
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| Molecular Formula |
C16H15D4NO8
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|---|---|
| Molecular Weight |
357.35
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| Related CAS # |
Penehyclidine;87827-02-9
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| Appearance |
Light brown to gray solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7984 mL | 13.9919 mL | 27.9838 mL | |
| 5 mM | 0.5597 mL | 2.7984 mL | 5.5968 mL | |
| 10 mM | 0.2798 mL | 1.3992 mL | 2.7984 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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