| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The SNAP-tag protein, a modified version of human O6-alkylguanine-DNA alkyltransferase (hAGT). The benzylguanine moiety of Alkyne-PEG5-SNAP reacts specifically and rapidly with the active site cysteine of the SNAP-tag, forming a stable covalent bond. The alkyne group remains free for secondary conjugation.
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| ln Vitro |
In test tube reactions, Alkyne-PEG5-SNAP reacts with purified SNAP-tag fusion proteins with high specificity and efficiency, resulting in covalent labeling of the protein at the active site. The alkyne group is available for copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.
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| ln Vivo |
SNAP-tag expressing cells are treated with Alkyne-PEG5-SNAP, and the reaction is complete within 15-30 minutes. The PEG5 linker reduces steric hindrance and improves solubility. The alkyne handle is then used for subsequent click chemistry conjugation with azide-fluorophores or other probes.
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| Enzyme Assay |
Non-cellular: Purified SNAP-tagged protein is incubated with Alkyne-PEG5-SNAP in reaction buffer (e.g., 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05% Tween-20) at room temperature for 15-30 minutes. Unreacted reagent is removed by size exclusion or dialysis. Labeling efficiency is assessed by mass spectrometry or SDS-PAGE with fluorescence scanning.
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| Cell Assay |
Cells expressing a SNAP-tag fusion protein of interest are cultured in complete media. Alkyne-PEG5-SNAP is added at 1-10 uM and incubated for 15-30 minutes at 37degC. After washing, cells are lysed or fixed. The alkyne handle is then reacted with an azide-fluorophore (e.g., azide-AF488) via CuAAC for 1-2 hours, enabling fluorescence visualization.
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| Animal Protocol |
Not applicable. Alkyne-PEG5-SNAP is not administered in vivo as a drug. It is a chemical tool for in vitro cell labeling and is not used for therapeutic purposes in animals. Its in vivo use would be limited by the requirement for SNAP-tag expression and copper-catalyzed click chemistry.
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| ADME/Pharmacokinetics |
The SNAP-tag reaction is rapid and irreversible in vitro (t1/2 ~1-2 minutes). Alkyne-PEG5-SNAP is stable in dry form and in solution for short-term storage at -20degC. The alkyne group is stable under physiological conditions for several hours, allowing for subsequent click chemistry.
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| Toxicity/Toxicokinetics |
No toxicity data for this specific reagent. The benzylguanine moiety is generally non-toxic at labeling concentrations (1-10 uM). Copper used in the click chemistry step can be cytotoxic; copper-free click chemistry may be required for live cell applications.
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| References | |
| Additional Infomation |
Alkyne-PEG5-SNAP is a click chemistry-based SNAP-tag substrate. The PEG5 linker increases aqueous solubility and reduces aggregation. It is used for protein labeling, live-cell imaging, pull-down assays, and protein-protein interaction studies. Not for therapeutic use.
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| Molecular Formula |
C27H36N6O7
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|---|---|
| Molecular Weight |
556.61
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~125 mg/mL (~224.57 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7966 mL | 8.9830 mL | 17.9659 mL | |
| 5 mM | 0.3593 mL | 1.7966 mL | 3.5932 mL | |
| 10 mM | 0.1797 mL | 0.8983 mL | 1.7966 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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