| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Allophycocyanin functions as a fluorescent label and does not target a specific biological receptor. It is used to conjugate to antibodies, streptavidin, or other biomolecules via chemical crosslinkers, enabling visualization of target proteins in cells and tissues by fluorescence.
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| ln Vitro |
In biochemical assays, APC is used as a tandem conjugate with other fluorophores (APC-Cy7, APC-Alexa Fluor 750) to extend emission into the near-infrared. Its bright fluorescence and high quantum yield make it ideal for flow cytometry-based cell sorting (FACS) and multiplexing assays.
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| ln Vivo |
Allophycocyanin itself is not administered in vivo as a drug. However, APC-conjugated antibodies or probes may be used in in vivo imaging studies in animal models to track tumor cells, immune cells, or other biomarkers. Its far-red emission allows for deeper tissue penetration.
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| Enzyme Assay |
In a typical conjugation reaction, APC is dissolved in a suitable buffer, and a crosslinker (e.g., SMCC, sulfo-SMCC) is used to activate the protein. An antibody or streptavidin is then added to form the conjugate. Excess reagents are removed by size exclusion chromatography.
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| Cell Assay |
Cells are fixed, permeabilized, and incubated with APC-conjugated antibodies or streptavidin in staining buffer. After washing, fluorescence is analyzed using a flow cytometer (excitation 633-640 nm, emission 660 nm) or fluorescence microscope. For live-cell imaging, cells are incubated with APC-conjugated probes under physiological conditions.
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| Animal Protocol |
In mouse xenograft models, APC-conjugated tumor-targeting antibodies are injected intravenously, and in vivo fluorescence imaging is performed at multiple time points. The APC signal (excitation ~640 nm, emission ~660 nm) is measured using an IVIS imaging system equipped with far-red/ NIR filters.
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| ADME/Pharmacokinetics |
APC in solution is stable at 4degC for days to weeks but is photosensitive. It should be stored in the dark. In vivo, APC-conjugates have variable half-lives depending on the carrier molecule, ranging from hours (free APC) to days (antibody-APC conjugates).
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| Toxicity/Toxicokinetics |
APC itself is non-toxic at typical concentrations used in staining (≤0.1-1 ug/mL). When conjugated to antibodies or nanoparticles, the safety profile depends on the conjugate, not the fluorophore. Standard laboratory safety practices are sufficient.
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| References | |
| Additional Infomation |
APC is one of the brightest fluorophores available for flow cytometry, with an extinction coefficient of ~700,000 cm-¹M-¹ and quantum yield ~0.68. It is commonly used in multicolor panels due to minimal spectral overlap with other common dyes (e.g., FITC, PE). Not for human therapeutic use.
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| Appearance |
Light blue to blue liquid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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