| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
BGSN3 targets SNAP-tag and H5 enzymes as substrates. SNAP-tag is a self-labeling protein tag derived from O6-alkylguanine-DNA alkyltransferase, commonly used in protein labeling and imaging applications. H5 is another enzyme that also processes BGSN3 as a substrate. The compound is a click chemistry reagent rather than a traditional drug target inhibitor, and its primary use is in chemical biology workflows for labeling and detection of tagged proteins.
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| ln Vitro |
SNAP-tag® is a potent technique that uses benzyl-guanine (BG) derivatives as substrates to label proteins and enzymes[1]. For the direct immobilization of these tags (SNAP-tag® and its somewhat thermally stable variant, SsOGT-H5) on solid surfaces, BGSN3 has shown to be an effective substrate[1].
In vitro, BGSN3 serves as a substrate for SNAP-tag and H5 enzymes, with IC50 values of 17.8 microM and 10 microM, respectively. These values indicate the concentration at which the compound occupies the active sites of these enzymes, enabling covalent attachment. The compound is not typically assessed for cell viability or proliferation effects, as it is a biochemical labeling reagent rather than a therapeutic agent. Its utility lies in enabling CuAAC or SPAAC reactions with alkyne-containing molecules for protein functionalization. |
| ln Vivo |
In vivo activity data for BGSN3 have not been reported. As a click chemistry reagent and SNAP-tag substrate, BGSN3 is designed for use in in vitro biochemical and cell-based labeling applications. It is not intended for in vivo administration in animal models, and no animal efficacy or pharmacokinetic studies are typically conducted for this type of chemical biology tool compound.
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| Enzyme Assay |
Binding studies for BGSN3 involve measuring its interaction with SNAP-tag and H5 enzymes. Typically, the activity of BGSN3 as a substrate is assessed by incubating the compound with purified SNAP-tag or H5 enzymes. The reaction progress can be monitored by measuring the covalent attachment of BGSN3 to the enzyme, often using fluorescence detection if the compound contains a fluorophore, or by mass spectrometry to confirm covalent modification. The IC50 values (17.8 microM for SNAP-tag, 10 microM for H5) are derived from such assays.
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| Cell Assay |
Cellular experiments for BGSN3 are conducted in cells expressing SNAP-tag fusion proteins. Cells are incubated with BGSN3, which contains an azide group, allowing the compound to covalently attach to the SNAP-tag. Subsequently, a second molecule containing an alkyne group (e.g., a fluorophore or affinity tag) can be conjugated via CuAAC or SPAAC click chemistry. This two-step labeling strategy enables specific and versatile protein labeling in live or fixed cells. Labeling efficiency can be assessed by fluorescence microscopy or flow cytometry.
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| Animal Protocol |
In vivo animal experiments are not conducted with BGSN3, as it is a chemical biology reagent designed for in vitro applications. There are no animal models or administration protocols described for BGSN3 in standard product literature. The compound is strictly used for protein labeling in biochemical and cellular contexts and not intended for therapeutic evaluation.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for BGSN3 are not available or applicable. This compound is a click chemistry reagent and SNAP-tag substrate used for in vitro protein labeling and detection applications. It is not designed or characterized for in vivo administration, and no ADME (absorption, distribution, metabolism, excretion) data are provided in standard product literature.
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| Toxicity/Toxicokinetics |
Toxicological data for BGSN3 are not extensively reported in standard product literature. As a research-use click chemistry reagent, typical safety assessments are not detailed. Standard laboratory safety precautions for handling chemical reagents should be observed, including working in a fume hood and using appropriate personal protective equipment such as gloves and lab coats.
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| References |
[1]. The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. J Enzyme Inhib Med Chem. 2021;36(1):85-97.
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| Additional Infomation |
BGSN3 has the molecular formula C17H19N9O2 and a molecular weight of 381.39. The CAS number is 2996069-91-9. It is a click chemistry reagent containing an azide functional group that enables CuAAC reactions with alkyne-bearing molecules and SPAAC reactions with DBCO or BCN groups. The compound is a good substrate for SNAP-tag and H5 enzymes. It is used in biochemical assay applications for protein labeling and detection, as well as for identifying modification sites in N-myristoylated and GPI-anchored proteins in blood-stage P. falciparum. It is for research use only.
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| Molecular Formula |
C17H19N9O2
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|---|---|
| Molecular Weight |
381.39
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| Appearance |
White to off-white solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (262.2 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6220 mL | 13.1099 mL | 26.2199 mL | |
| 5 mM | 0.5244 mL | 2.6220 mL | 5.2440 mL | |
| 10 mM | 0.2622 mL | 1.3110 mL | 2.6220 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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