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| 1mg |
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| 5mg |
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| 10mg |
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| Targets |
The compound targets proteins containing alkyne tags through its azide functional group. It is specifically utilized for identifying modification sites in N-myristoylation and GPI-anchored proteins in blood-stage Plasmodium falciparum. The integrated trypsin-cleavable Gly-Arg-Gly linker allows for the release of lipidated peptides after streptavidin enrichment, facilitating downstream mass spectrometry analysis. This design addresses the issue of biotin moiety suppressing peptide ionization in MS detection.
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| ln Vitro |
In vitro, the compound demonstrates high utility in chemical proteomics applications. Benchmark data from an Angewandte study show that an optimal cleavable design identified 38 high-confidence lipidated peptides, while this compound (identified as Reagent 5) serves as an essential negative control for linker geometry optimization. It has been validated in HEK293, HeLa, and MCF7 cells with >99% peptide identification confidence in the proper workflow.
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| ln Vivo |
In vivo activity data for Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA are not applicable or reported. This compound is a chemical biology reagent designed for in vitro proteomics applications, specifically for protein labeling and enrichment. It is not intended for in vivo administration in animal models, and no animal efficacy studies have been conducted or described for this click chemistry reagent.
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| Enzyme Assay |
Binding studies for this compound utilize the high-affinity biotin-streptavidin interaction. The biotin moiety allows for capture on streptavidin-coated beads or surfaces. The azide group enables covalent conjugation to alkyne-tagged biomolecules via click chemistry. Binding can be assessed by incubating the compound with alkyne-modified proteins, followed by streptavidin pull-down and analysis by SDS-PAGE or LC-MS/MS to confirm successful conjugation and capture.
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| Cell Assay |
Cellular experiments involve treating cell lysates or live cells expressing alkyne-tagged proteins (via metabolic labeling) with Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA. The click reaction (CuAAC or SPAAC) covalently attaches the biotin tag to target proteins. Cells are then lysed, and biotinylated proteins are captured on streptavidin beads, eluted (often by trypsin digestion of the Gly-Arg-Gly linker), and analyzed by mass spectrometry for protein identification and modification site mapping.
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| Animal Protocol |
In vivo animal experiments are not conducted with this compound, as it is strictly a chemical biology reagent for in vitro applications. There are no animal models or administration protocols described for Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA in standard product literature or scientific documentation for the parent compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA are not available or applicable. This compound is a click chemistry reagent intended for use in in vitro chemical proteomics and protein labeling workflows. It is not designed or characterized for in vivo administration, and no ADME (absorption, distribution, metabolism, excretion) data are provided in standard product literature.
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| Toxicity/Toxicokinetics |
Toxicological data for Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA are not extensively reported in standard product literature. As a research-use click chemistry reagent, typical safety assessments are not detailed. Standard laboratory safety precautions for handling chemical reagents should be observed, including working in a fume hood and using appropriate personal protective equipment such as gloves and lab coats.
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| References | |
| Additional Infomation |
Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA has the molecular formula C38H66F3N13O11S and a molecular weight of 970.1 g/mol. It contains an azide group for CuAAC and SPAAC click reactions, a trypsin-cleavable Gly-Arg-Gly linker that enables MS-friendly release of captured peptides, and a PEG3 spacer to enhance solubility in aqueous buffers. The compound is typically stored at -20degC, sealed, away from moisture.
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| Molecular Formula |
C38H66F3N13O11S
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| Molecular Weight |
970.07
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~103.09 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0309 mL | 5.1543 mL | 10.3085 mL | |
| 5 mM | 0.2062 mL | 1.0309 mL | 2.0617 mL | |
| 10 mM | 0.1031 mL | 0.5154 mL | 1.0309 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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