| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
Cysteine thiols (-SH groups) on proteins or peptides (via iodoacetamide alkylation); desthiobiotin binds to streptavidin/avidin.
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|---|---|
| ln Vitro |
The iodoacetamide group reacts specifically with reduced thiols at pH 7.5-8.5 to form a stable thioether linkage; desthiobiotinylated proteins are captured by streptavidin affinity columns and eluted under mild conditions with free biotin.
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| ln Vivo |
No in vivo activity data are available; the compound is an in vitro proteomics tool for peptide mapping, phosphopeptide analysis, and mass spectrometry applications.
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| Enzyme Assay |
Streptavidin binding affinity (Ka~10^-11 M) is characterized by competitive displacement assays; labeling efficiency is assessed by incubating cysteine-containing peptides with the reagent, then analyzing desthiobiotinylation by MALDI-TOF MS.
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| Cell Assay |
Cell lysates are treated with desthiobiotin PEO iodoacetamide to label cysteine residues; labeled peptides are fractionated by affinity capture, eluted with biotin, and analyzed by LC-MS/MS for proteome-wide cysteine profiling.
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| Animal Protocol |
In vivo protocols are not applicable; tissue homogenates can be labeled ex vivo to study cysteine modifications in disease models.
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| ADME/Pharmacokinetics |
PK studies are not performed; the reagent is stable as a powder at -20degC for up to 3 years but is unstable in solution, requiring immediate use after preparation.
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| Toxicity/Toxicokinetics |
Toxicity data are not available; the compound contains an alkylating agent and should be handled with standard chemical safety precautions; it is for research use only.
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| References |
[1]. Patrick R A Zanon, et al. Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome. Angew Chem Int Ed Engl. 2020 Feb 10;59(7):2829-2836.
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| Additional Infomation |
This product is a proteomics research tool; it has no clinical trial status or regulatory approval as a therapeutic agent.
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| Molecular Formula |
C21H38IN5O6
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|---|---|
| Molecular Weight |
583.46
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| Appearance |
Light yellow to yellow solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7139 mL | 8.5696 mL | 17.1391 mL | |
| 5 mM | 0.3428 mL | 1.7139 mL | 3.4278 mL | |
| 10 mM | 0.1714 mL | 0.8570 mL | 1.7139 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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