| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Diquat acts as a herbicide by generating reactive oxygen species (ROS) in plant cells; the labeled variant is not intended for pharmacological target identification.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an impact on a drug's pharmacokinetics and metabolic profile, it has drawn attention [1].
Diquat dibromide has an IC50 of 60 mg/L in the Allium root growth inhibition test; the deuterated analog is chemically similar and shares this activity. |
| ln Vivo |
In vivo activity matches the unlabeled compound: Diquat is a fast-acting contact herbicide that causes rapid desiccation and defoliation in plants; the labeled version is used primarily for internal standardization.
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| Enzyme Assay |
Stable heavy isotope-labeled compounds are used as tracers for quantitation, detected via LC-MS/MS analysis; samples are spiked with Diquat-d4 as an internal standard, extracted, and analyzed on a suitable chromatographic column.
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| Cell Assay |
Cells (e.g., Allium root cells or mammalian cell lines) are exposed to Diquat dibromide at varying concentrations; cell viability or growth inhibition is assessed to determine IC50 values; the labeled analog serves as internal standard in quantification experiments.
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| Animal Protocol |
Animal models are used to study Diquat toxicity; tissues are collected and analyzed for Diquat content using Diquat-d4 as an internal standard in LC-MS-based bioanalytical methods.
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| ADME/Pharmacokinetics |
The pharmacokinetic profile of Diquat-d4 dibromide matches that of the unlabeled form; as a quaternary ammonium compound, Diquat is poorly absorbed from the gastrointestinal tract and is primarily excreted unchanged in feces.
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| Toxicity/Toxicokinetics |
Diquat dibromide is a known toxic herbicide; acute poisoning in mammals can cause severe gastrointestinal and renal toxicity; the deuterated analog is expected to have a similar toxicological profile and must be handled with caution.
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| References |
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| Additional Infomation |
This product is a stable isotope-labeled analytical standard for research use only; it is not an approved drug and has no clinical trial or marketing authorization for human therapeutic applications.
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| Molecular Formula |
C12H8D4BR2N2
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|---|---|
| Molecular Weight |
348.07
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| Related CAS # |
Diquat dibromide;85-00-7
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8730 mL | 14.3649 mL | 28.7299 mL | |
| 5 mM | 0.5746 mL | 2.8730 mL | 5.7460 mL | |
| 10 mM | 0.2873 mL | 1.4365 mL | 2.8730 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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