| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
Isocitrate lyase 1 (ICL1); a key enzyme in the glyoxylate shunt and methylcitrate cycle, essential for bacterial survival under nutrient-limited conditions.
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|---|---|
| ln Vitro |
ICL1 has dual activity for both threo-D(s)L(s)-isocitrate (ICA) and DL-threo-2-methylisocitrate (MICA); For ICA, Km = 188 microM, kcat = 5.24 s-1, kcat/Km = 2.79 × 10^4 M-1 s-1; For MICA, kcat/Km = 1.74 × 10^3 M-1 s-1.
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| ln Vivo |
No direct in vivo activity; used as a substrate probe to study the glyoxylate and methylcitrate cycles in M. tuberculosis; provides insights into metabolic disorders, microbial physiology, and potential antimicrobial targets.
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| Enzyme Assay |
Enzymatic activity: purified recombinant ICL1 enzyme (0.1-1 microM) is incubated with DL-threo-2-methylisocitrate (0-2 mM) in reaction buffer; product formation (pyruvate or glyoxylate) is measured spectrophotometrically at 324 nm via a coupled enzyme assay.
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| Cell Assay |
Mycobacterium tuberculosis cultures are grown in media containing DL-threo-2-methylisocitrate as a carbon source; bacterial growth is monitored by OD600; ICL1 activity in cell lysates is measured; metabolites are analyzed by HPLC or LC-MS.
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| Animal Protocol |
Mice infected with M. tuberculosis are treated with compounds modulating ICL1 activity; tissues collected; ICL1 substrate utilization assessed; detailed protocol not defined.
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| ADME/Pharmacokinetics |
No PK data; DL-threo-2-methylisocitrate is a metabolic intermediate and substrate; cellular uptake and metabolism are rapid; half-life likely short due to rapid utilization.
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| Toxicity/Toxicokinetics |
No toxicity data; DL-threo-2-methylisocitrate is a metabolite analog with minimal toxicity; standard laboratory safety precautions are sufficient; for research use only.
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| References |
[1]. Gould TA, et al. Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis. Mol Microbiol. 2006 Aug;61(4):940-7.
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| Additional Infomation |
DL-threo-2-methylisocitrate sodium is a research-grade biochemical tool; not a drug; no clinical trial or marketing approval status; used to investigate bacterial and mammalian metabolic pathways, enzyme kinetics, and regulation of propionate metabolism.
|
| Molecular Formula |
C14H14NA6O14
|
|---|---|
| Molecular Weight |
544.19
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| Related CAS # |
DL-threo-2-methylisocitrate;71183-66-9
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~100 mg/mL (~183.76 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8376 mL | 9.1880 mL | 18.3759 mL | |
| 5 mM | 0.3675 mL | 1.8376 mL | 3.6752 mL | |
| 10 mM | 0.1838 mL | 0.9188 mL | 1.8376 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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