| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
PEGs
Iodo-PEG7-alcohol does not target a specific biological receptor; it is a chemical linker. The iodo group serves as a leaving group in nucleophilic substitution reactions (SN2) with thiols, amines, or other nucleophiles. The alcohol group can be further functionalized (e.g., activated as a carbonate or succinimidyl ester) for conjugation to proteins or small molecules. |
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| ln Vitro |
A linker separates the two ligands that make up PROTACs; one ligand is for an E3 ubiquitin ligase, and the other is for the target protein. Target proteins are selectively degraded by PROTACs by taking advantage of the intracellular ubiquitin-proteasome system[1].
Iodo-PEG7-alcohol is not used in enzyme/receptor binding assays. Its reactivity is evaluated in cell-free chemical reactions. For example, it reacts with thiol-containing compounds (e.g., cysteine, glutathione) in aqueous buffer at pH 7-9 to form a stable thioether linkage. Reaction progress is monitored by HPLC or LC-MS. No biological activity is intended or observed. |
| ln Vivo |
In cell culture, Iodo-PEG7-alcohol (typically 1-100 uM) can be used to label surface-exposed thiols on proteins if the iodo group is reactive. However, it is generally not used directly on cells; instead, it is first conjugated to a targeting ligand (e.g., antibody) via the alcohol group after activation. The conjugate then binds to the target cell. The compound itself has no inherent cytotoxicity at working concentrations.
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| Enzyme Assay |
To test reactivity, a cell-free assay is performed: Iodo-PEG7-alcohol (10-100 uM) is incubated with a thiol-containing model compound (e.g., N-acetylcysteine, 1 mM) in PBS (pH 7.4) at 37degC for 1-24 hours. Aliquots are taken and analyzed by HPLC or LC-MS to determine the conversion rate. The reaction is typically complete within a few hours. Control reactions without thiol show no change. The reaction follows second-order kinetics.
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| Cell Assay |
If Iodo-PEG7-alcohol is conjugated to a cell-targeting ligand (e.g., an RGD peptide), the conjugate is incubated with target cells (e.g., integrin-expressing cancer cells) at 1-10 uM for 1-4 hours. Cellular binding is assessed by flow cytometry using a fluorescent tag attached via the alcohol group (after activation). Alternatively, the conjugate can deliver a payload (e.g., drug) to cells, and cytotoxicity is measured by MTT. The PEG7 spacer enhances solubility and reduces steric hindrance.
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| Animal Protocol |
Iodo-PEG7-alcohol itself is rarely administered in vivo. Instead, it is used ex vivo to synthesize conjugates that are then administered. For example, an ADC made using this linker is given intravenously in tumor-bearing mice at 1-10 mg/kg. The linker is stable in circulation; the iodine atom is displaced only upon intracellular thiol exposure. The alcohol end can be used for further conjugation. PK studies are performed on the final conjugate, not the linker alone.
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| ADME/Pharmacokinetics |
As a small PEGylated molecule, Iodo-PEG7-alcohol has a molecular weight of approximately 450-500 Da. It is soluble in water and common organic solvents (DMSO, DMF). The iodo group is reactive and may undergo hydrolysis at high pH or in the presence of light. The compound should be stored at -20degC under inert atmosphere, protected from light. The PEG7 chain imparts hydrophilicity and reduces non-specific protein binding.
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| Toxicity/Toxicokinetics |
Iodo-PEG7-alcohol is considered a chemical reagent with low acute toxicity. The iodo group can be alkylating, so the compound should be handled with care (potential skin/eye irritant). In cellular assays at ≤100 uM, no significant cytotoxicity has been reported. For in vivo use of conjugates, the linker itself is typically non-toxic at the doses delivered. Standard lab safety (gloves, goggles, fume hood) is recommended.
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| References |
[1]. Nalawansha DA, et al. PROTACs: An Emerging Therapeutic Modality in Precision Medicine. Cell Chem Biol. 2020;27(8):998-985.
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| Additional Infomation |
Iodo-PEG7-alcohol is a research chemical used as a linker in the synthesis of ADCs, PROTACs, and other bioconjugates. The PEG7 spacer provides a hydrophilic, flexible bridge that improves solubility and reduces aggregation. The iodine atom is an excellent leaving group for thiol-alkylation. The alcohol group can be converted to an active ester (NHS) or other functional groups. This product is for laboratory use only and is not approved for human therapeutic use.
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| Molecular Formula |
C14H29IO7
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|---|---|
| Molecular Weight |
436.28
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| Appearance |
Colorless to light yellow liquid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2921 mL | 11.4605 mL | 22.9211 mL | |
| 5 mM | 0.4584 mL | 2.2921 mL | 4.5842 mL | |
| 10 mM | 0.2292 mL | 1.1461 mL | 2.2921 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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