| Size | Price | Stock | Qty |
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| 50mg |
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| Other Sizes |
| Targets |
IR-806 does not target a specific biological receptor; it is a free dye that can be conjugated to targeting ligands (antibodies, peptides) or used as a non-specific contrast agent. When conjugated, the conjugate targets the desired biomarker (e.g., tumor-associated antigens). The dye itself accumulates passively in tissues via enhanced permeability and retention (EPR) effect.
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| ln Vitro |
In DMSO (10 mL) solution, EDC/NHS (1×10-4 mol) was used to activate IR-806 (0.1 mg) and folic acid (0.02 mg). After 30 minutes, the 5 mg UCN-containing PL-PEG-NH2-coated UCN solution was added, and it was mixed for 4 hours at room temperature. In order to obtain nanocrystals (UCNs), which were utilized to create nanoprobes, the reaction product was purified by centrifugation at 10,000 rpm for five minutes. The UCNs were then suspended in five milliliters of DMSO [2].
In cell-free systems, IR-806 is characterized by its absorption and emission spectra, quantum yield, and photostability. The molar extinction coefficient is high (>200,000 M-¹cm-¹). The dye has good solubility in organic solvents and moderate solubility in aqueous buffers (often with sulfonate groups). It is not used in enzyme/receptor binding assays unless conjugated. |
| ln Vivo |
In cell culture, free IR-806 (1-20 uM) can be taken up by cells via endocytosis, but it shows low non-specific binding. When conjugated to an antibody (e.g., anti-EGFR), the labeled antibody specifically stains target-positive cells, visualized by NIR fluorescence microscopy. Conjugation does not significantly alter the dye's spectral properties. No direct biological activity (e.g., cytotoxicity) is expected at imaging concentrations.
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| Enzyme Assay |
For in vitro characterization, IR-806 is dissolved in DMSO or PBS. UV-Vis-NIR absorption is recorded with a spectrophotometer, and fluorescence emission is recorded with a fluorometer. Quantum yield is calculated using a reference dye (e.g., ICG). Photostability is tested by continuous illumination and monitoring intensity decay over time. For conjugation, the dye's reactive group (e.g., NHS ester if present) is used to label amine-containing molecules.
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| Cell Assay |
Cells expressing a target antigen (e.g., EGFR) are incubated with IR-806-labeled antibody (1-10 ug/mL) for 30-60 minutes at 4degC. After washing, cellular fluorescence is measured by flow cytometry or NIR fluorescence microscopy. Non-specific binding is controlled with an isotype control-labeled dye or by pre-blocking with excess unlabeled antibody. The dye's fluorescence intensity is proportional to target expression level.
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| Animal Protocol |
IR-806 is administered intravenously in tumor-bearing mice (0.5-2 mg/kg for the dye alone or 0.1-0.5 mg/kg for the conjugate). NIR fluorescence imaging is performed at various time points (0-48 hours). The dye accumulates in tumors via EPR (free dye) or specific targeting (conjugate). The signal-to-background ratio is calculated. Ex vivo organ imaging confirms tumor-specific uptake. IR-806 allows image-guided tumor resection due to its deep tissue penetration (up to 1 cm).
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| ADME/Pharmacokinetics |
IR-806 has a molecular weight of approximately 1000 Da. It is cleared primarily via hepatobiliary excretion (feces) due to its size and hydrophobicity. The plasma half-life in mice is typically 1-2 hours for the free dye. Conjugation to antibodies extends the half-life to days. The dye is stable in plasma for several hours. It has low quantum yield in water but improves in lipid-rich environments; this property enhances tumor imaging.
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| Toxicity/Toxicokinetics |
IR-806 is well-tolerated at imaging doses (≤2 mg/kg in mice). No acute toxicity or body weight loss has been observed. High doses may cause mild liver enzyme elevation due to dye accumulation in hepatocytes. The dye is not known to be genotoxic or carcinogenic. For research use only; no clinical safety data are available. Standard lab safety practices should be followed. Avoid prolonged exposure to light.
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| References | |
| Additional Infomation |
IR-806 is a research-grade near-infrared dye used for in vivo fluorescence imaging. It is not approved for clinical use. The dye's long-wavelength excitation and emission minimize tissue autofluorescence and allow deep tissue imaging. It is commonly used in preclinical tumor imaging, lymph node mapping, and surgical guidance. The compound may be supplied as a powder; store at -20degC protected from light. Different reactive derivatives (NHS, maleimide) are available for conjugation.
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| Molecular Formula |
C37H44CLN2NAO6S2
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|---|---|
| Molecular Weight |
735.33
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| Appearance |
Green to dark green solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~125 mg/mL (~169.99 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3599 mL | 6.7997 mL | 13.5993 mL | |
| 5 mM | 0.2720 mL | 1.3599 mL | 2.7199 mL | |
| 10 mM | 0.1360 mL | 0.6800 mL | 1.3599 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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