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| Targets |
As a culture medium, LB Broth Base does not have molecular targets. Its components (peptone, yeast extract, sodium chloride) provide nutrients-carbon sources, nitrogen sources, amino acids, vitamins, and inorganic salts-to support bacterial growth.
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| ln Vitro |
LB Broth Base is a standard medium for bacterial culture, used extensively in molecular biology for preparing competent cells, propagating plasmid DNA, performing protein expression, and conducting various microbiological assays. It supports robust growth of E. coli and many other bacterial species.
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| ln Vivo |
LB Broth Base is used in vivo only in the context of administering bacteria grown in this medium to animals for infection models. The medium itself is not administered to animals as a therapeutic agent. The growth characteristics of bacteria cultured in LB medium are well-established and reproducible.
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| Enzyme Assay |
LB Broth Base itself is a complex mixture and not used in receptor binding assays. However, it can be utilized as a component in non-cell enzyme assays when recombinant proteins are produced in E. coli. For example, E. coli cells are cultured in LB broth to express a target protein of interest, which is subsequently purified and used in cell-free binding assays such as surface plasmon resonance, ELISA, or fluorescence polarization. The medium‘s composition ensures optimal bacterial growth and high protein yields for such downstream applications.
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| Cell Assay |
LB Broth Base is not used in cell culture experiments with eukaryotic cells. Its primary application is in bacterial cell culture. For bacterial culture, the powdered LB Broth Base is reconstituted in distilled water (typically 25 g of powder per liter) and autoclaved at 121degC for 15 minutes to achieve sterility. For solid media, 1.5% agar (15 g/L) is added before autoclaving. E. coli cells (e.g., DH5alpha, BL21(DE3)) are inoculated from glycerol stocks or agar plates into liquid LB medium (5-10 mL) and incubated at 37degC with shaking at 200-250 rpm for 8-16 hours. Cell growth is monitored by measuring optical density at 600 nm (OD600). When OD600 reaches 0.4-0.6 for expression cultures, protein expression is induced by adding IPTG (isopropyl beta-D-1-thiogalactopyranoside, 0.1-1 mM). For transformation selection, ampicillin (100 microg/mL), kanamycin (50 microg/mL), or other antibiotics are added to LB medium. For preparation of chemically competent cells, cells are grown in LB to mid-log phase (OD600 ~0.4), then harvested and treated with CaCl2.
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| Animal Protocol |
LB Broth Base is not directly administered to animals. It is used in the preparation of bacterial inocula for animal infection models. For in vivo infection studies, bacterial strains (e.g., E. coli, Salmonella, Listeria) are cultured overnight in LB broth. The following day, cultures are diluted 1:100 into fresh LB broth and grown to mid-logarithmic phase (OD600 ~0.5-0.6). Bacteria are pelleted by centrifugation, washed with sterile PBS, and resuspended in PBS or appropriate diluent. The bacterial suspension is then administered to mice or rats via various routes including intravenous (tail vein), intraperitoneal, or oral gavage (typically 10⁶-10⁸ CFU per animal). For lethal infection models, a predetermined dose that kills 50-100% of animals is used to evaluate the efficacy of antimicrobial compounds. Blood and tissue samples are collected at various time points post-infection, plated onto LB agar plates, and incubated overnight at 37degC to enumerate viable bacteria (CFU/g tissue).
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| ADME/Pharmacokinetics |
LB Broth Base is a non-therapeutic growth medium and does not undergo pharmacokinetic evaluation. The individual components (peptone, yeast extract, NaCl) are nutrients that are absorbed, metabolized, or excreted according to normal physiological pathways when administered orally as part of animal feed. Peptones and yeast extracts are digested into amino acids and small peptides, absorbed in the small intestine, and incorporated into protein synthesis or catabolized. Sodium chloride is absorbed and participates in electrolyte balance. For parenteral administration (as may occur during bacterial infection when medium components are co-administered with bacteria), these components are rapidly cleared from the bloodstream. Specific pharmacokinetic parameters for LB Broth Base components in the context of animal infection models are not available.
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| Toxicity/Toxicokinetics |
LB Broth Base is generally regarded as safe for its intended use as a bacterial culture medium. The individual components-peptone (protein hydrolysate), yeast extract, and sodium chloride-are all food-grade materials with low toxicity profiles. Upon autoclaving, the medium is sterile and free of viable microorganisms. Accidental ingestion or injection of LB Broth Base in a laboratory setting could cause mild gastrointestinal disturbance or injection-site irritation. No significant acute or chronic toxicity is associated with LB Broth Base. Standard laboratory precautions for handling powdered media should be followed to avoid inhalation of dust particles, which may cause respiratory irritation.
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| References |
[1]. Jia Y, et al. Hydrogel dressing integrating FAK inhibition and ROS scavenging for mechano-chemical treatment of atopic dermatitis. Nat Commun. 2023 Apr 29;14(1):2478.
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| Additional Infomation |
LB Broth Base, also known as Luria-Bertani Base or LB medium, is one of the most widely used media in molecular biology and microbiology. The standard composition per liter of LB medium is: 10 g peptone (provides nitrogen, amino acids, and peptides), 5 g yeast extract (provides vitamins and trace elements), and 5-10 g sodium chloride (provides electrolytes and maintains osmotic balance). LB medium supports fast growth of E. coli and is used for competent cell preparation (both chemically competent and electrocompetent cells), plasmid DNA isolation (miniprep, midiprep, maxiprep), recombinant protein expression in systems such as pET vectors in BL21(DE3) cells, phage propagation, and routine bacterial maintenance and storage. Several variants exist including Miller‘s LB (10 g/L NaCl) and Lennox LB (5 g/L NaCl). LB Broth Base is strictly a research tool and is not approved for human consumption or clinical use.
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :≥ 100 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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