| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
MeCY5-NHS ester does not target a specific biological receptor or enzyme; its NHS ester moiety reacts chemically with primary amine groups (-NH2) present on lysine residues of proteins or the N-terminus of peptides.
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|---|---|
| ln Vitro |
Not available. As a reactive dye, its activity is assessed by labeling efficiency rather than biological activity. The triethylamine salt form improves solubility and stability in aqueous buffers compared to the potassium salt variant.
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| ln Vivo |
Not available. As an in vitro labeling reagent, MeCY5-NHS ester is not intended for in vivo pharmacological studies; however, Cy5-labeled biomolecules are used for in vivo imaging to track distribution and biodistribution.
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| Enzyme Assay |
Not available. Standard NHS ester labeling protocols involve dissolving the dye in anhydrous DMSO or DMF, mixing with protein solution in bicarbonate buffer (pH 8.0-8.5) at molar ratios of 5:1 to 20:1, incubating at room temperature for 1-2 hours, then removing excess dye by desalting or dialysis.
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| Cell Assay |
Not available. For cellular labeling, typical protocols involve incubating cells with NHS ester-labeled antibodies or probes at concentrations of 1-10 microg/mL in PBS for 30-60 minutes at 4degC or room temperature, followed by washing and analysis by flow cytometry or fluorescence microscopy.
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| Animal Protocol |
Not available. For in vivo imaging, Cy5-labeled conjugates (typically antibodies or nanoparticles) are administered intravenously at doses of 1-10 mg/kg, and fluorescence signals are monitored using near-infrared imaging systems at various time points post-injection.
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| ADME/Pharmacokinetics |
Not available. As a small molecule dye, triethylamine salt formulations typically exhibit improved aqueous solubility. The NHS ester group is rapidly hydrolyzed in aqueous environments, with a half-life of minutes to hours depending on pH and temperature.
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| Toxicity/Toxicokinetics |
Not available. Cyanine dyes generally have low toxicity profiles at typical labeling concentrations (uM range). The NHS ester is reactive but hydrolysis yields non-toxic byproducts. No specific toxicological data for this compound are reported.
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| References | |
| Additional Infomation |
Sulfo-Cyanine5 NHS ester triethylamine is a research-grade fluorescent label with no intrinsic pharmacological activity. It has not undergone clinical trials nor received regulatory approval for therapeutic use. This product is strictly limited to laboratory research applications including immunofluorescence, flow cytometry, and fluorescence resonance energy transfer (FRET) assays.
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| Molecular Formula |
C42H56N4O10S2
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|---|---|
| Molecular Weight |
841.04
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| Related CAS # |
Sulfo-CY5-NHS ester potassium;2230212-27-6
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| Appearance |
Brown to reddish brown solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~83.33 mg/mL (~99.08 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1890 mL | 5.9450 mL | 11.8900 mL | |
| 5 mM | 0.2378 mL | 1.1890 mL | 2.3780 mL | |
| 10 mM | 0.1189 mL | 0.5945 mL | 1.1890 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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