| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Targets |
Aminoglycoside
Puromycin-d3 targets the ribosome. The non-deuterated parent, Puromycin, specifically inhibits peptidyl transferase activity in both prokaryotic and eukaryotic ribosomes by mimicking the 3'-end of aminoacyl-tRNA. It is incorporated into the growing polypeptide chain, causing premature chain termination and disrupting protein synthesis. The deuterium labeling does not alter the mechanism of action but serves as an internal standard for quantification. |
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantification throughout the drug development process. Due to its potential to alter the pharmacokinetic and metabolic characteristics of medications, deuteration has drawn attention[1].
Puromycin dihydrochloride (the non-deuterated parent) is a potent inhibitor of protein synthesis. The deuterated version is not used for biological activity assessment; it is used as an analytical standard. Stable heavy isotopes have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Puromycin-d3 can be used as an internal standard for LC-MS/MS quantitation. |
| ln Vivo |
Puromycin-d3 is not administered in vivo for therapeutic purposes. Its non-deuterated parent, Puromycin, is an antibiotic that inhibits protein synthesis. The compound is used in research as a selection agent for cells expressing the puromycin resistance gene. Puromycin-d3 serves as an analytical standard for PK studies of Puromycin in animal models, allowing accurate quantification of the parent drug in biological matrices.
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| Enzyme Assay |
Puromycin-d3 is not used in an enzyme binding assay; it is an internal standard for LC-MS/MS. A stock solution is prepared in methanol (1 mg/mL). For LC-MS/MS analysis, a fixed concentration of Puromycin-d3 (e.g., 10-100 ng/mL) is added to each biological sample (e.g., plasma, cell lysate). Samples are extracted and injected onto an LC-MS/MS system. Puromycin is detected in positive ion mode (MRM). The d3 internal standard has a mass shift of +3 Da.
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| Cell Assay |
Puromycin-d3 is not used in typical cell-based assays. The non-deuterated parent, puromycin, is used as a selection agent. Cells expressing the pac gene are cultured in medium containing puromycin (0.5-10 ug/mL). Resistant cells survive and proliferate. The optimal selection concentration is determined by kill curve experiments. Selection efficiency is confirmed by staining with crystal violet. The deuterated version is not used in these assays.
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| Animal Protocol |
Puromycin-d3 is used as an internal standard for PK studies of puromycin. A typical protocol: Male SD rats are administered an intravenous or intraperitoneal dose of puromycin dihydrochloride (1-10 mg/kg). Blood samples are collected at various time points. A fixed concentration of Puromycin-d3 is added to each plasma sample. Samples are processed and analyzed by LC-MS/MS. PK parameters are calculated using non-compartmental analysis.
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| ADME/Pharmacokinetics |
Puromycin-d3 has a molecular weight of 474.53 and the molecular formula C22H26D3N7O5. It is a solid with white to off-white color. The product should be stored at -20degC, sealed and protected from moisture. Stable heavy isotopes have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process.
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| Toxicity/Toxicokinetics |
No specific toxicity data for the deuterated version are available. The non-deuterated parent, puromycin, is toxic and inhibits protein synthesis in both prokaryotic and eukaryotic cells. It causes cell death in rapidly dividing cells and is not suitable for in vivo therapeutic use. As an analytical standard, the deuterated version is used in trace amounts and does not pose additional safety risks beyond standard chemical handling.
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| References | |
| Additional Infomation |
Puromycin-d3 (CL13900-d3) is a research-grade stable isotope-labeled compound used as an internal standard for LC-MS/MS quantification of Puromycin. The parent compound, Puromycin, is an aminoglycoside antibiotic that inhibits protein synthesis. This product is for research use only and is not for human therapeutic applications.
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| Molecular Formula |
C22H26D3N7O5
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| Molecular Weight |
474.53
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1073 mL | 10.5367 mL | 21.0735 mL | |
| 5 mM | 0.4215 mL | 2.1073 mL | 4.2147 mL | |
| 10 mM | 0.2107 mL | 1.0537 mL | 2.1073 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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