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| 1mg |
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| 5mg |
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| 10mg |
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| Targets |
CPNE7 (Copine-7) protein functional domain. Selcopintide acetate is a synthetic peptide that mimics the activity of full-length CPNE7 by binding to its molecular targets involved in odontoblast differentiation and dentin formation. It acts as a dentin production promoter and is used in dental and hard tissue research. The peptide sequence is KYKQKRRSYK (human CPNE7 residues 344-353).
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| ln Vitro |
Odontoblasts (MDPC-23 cells) are directly penetrated by selcopintide (Cpne7-DP) acetate[1]. The dspp promoter activity is increased in a dose-dependent manner by selcopintide(1, 10 μg) acetate [1].
In vitro, Selcopintide acetate directly penetrates into odontoblastic (MDPC-23) cells. It highly reproduces the in vitro effects of CPNE7 by upregulating odontoblast marker genes, including DSPP (dentin sialophosphoprotein) and Nestin. The peptide promotes odontoblast differentiation and dentin formation, making it a valuable tool for dental regeneration research. |
| ln Vivo |
In both shallow and deep cavity models, secopintide (Cpne7-DP; 10 µg combined with human DPC cells in 0.5% fibrin gel and implanted subcutaneously) acetate stimulates the development of dentin-like tissue and the closure of tubular dentin and dentinal tubules. Regeneration [1].
In vivo efficacy data for Selcopintide acetate are limited. As a peptide that promotes odontoblast differentiation and dentin formation, it is expected to have applications in dental pulp regeneration and repair of dentin defects. The peptide likely promotes reparative dentin formation in animal models of tooth injury. Further in vivo studies are needed to evaluate its potential for clinical translation in restorative dentistry. |
| Enzyme Assay |
Non-cell-based binding assay: For peptide characterization, mass spectrometry and HPLC are used to verify sequence identity and purity. Binding to its target protein(s) is typically studied by pull-down assays using biotinylated Selcopintide acetate immobilized on streptavidin beads, incubated with cell lysates from odontoblastic cells. Bound proteins are identified by mass spectrometry to confirm interaction with CPNE7-related pathways.
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| Cell Assay |
Cell-based activity assay protocol: MDPC-23 odontoblastic cells are seeded in 6-well plates (2×10⁵ cells/well) and cultured in differentiation medium. Selcopintide acetate is added at concentrations of 1-100 uM for 24-72 hours. For penetration studies, cells are treated with fluorescently labeled Selcopintide and visualized by confocal microscopy. Gene expression is assessed by qRT-PCR for DSPP, Nestin, Runx2, and other odontoblast markers. Protein expression is confirmed by Western blot.
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| Animal Protocol |
Animal/Disease Models: Immunocompromised mice (NIH-bg-nu-xid)[1]
Doses: 10 µg Route of Administration: subcutaneous (sc) transplantation in a 0.5% fibrin gel Experimental Results: Promotes dentin-like tissue formation. Promotes the regeneration of tubular dentin and dentinal tubule occlusion in shallow and deep cavity models. Dental injury repair model (suggested): Male Wistar rats (8-10 weeks old) undergo dental pulp exposure under anesthesia. Selcopintide acetate is applied directly to the exposed pulp at doses of 10-100 ug per tooth. The cavity is sealed with dental cement. Animals are euthanized at 1-4 weeks post-treatment. Teeth are extracted, fixed, decalcified, and processed for histological analysis. Dentin bridge formation is assessed by H&E and Masson‘s trichrome staining. Odontoblast marker expression is evaluated by immunohistochemistry. |
| ADME/Pharmacokinetics |
Pharmacokinetic data for Selcopintide acetate are limited. As a synthetic peptide (MW 1444.7, acetate salt), it has limited oral bioavailability and is typically applied topically or injected locally for dental applications. The peptide directly penetrates into odontoblastic cells when applied to the dental pulp. Stability and half-life in biological fluids should be evaluated if systemic administration is considered. Store at 2-8degC for short-term or -20degC for long-term storage.
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| Toxicity/Toxicokinetics |
Toxicity data for Selcopintide acetate are limited. The peptide is intended for research use only and not for human therapeutic applications. In vitro studies show direct cellular penetration and functional effects at micromolar concentrations. No significant cytotoxicity has been reported at effective concentrations. Standard safety precautions for handling peptides should be observed. Purity ≥95%.
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| References |
[1]. Lee YS, et al. Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide. Materials (Basel). 2020;13(20):4618. Published 2020 Oct 16.
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| Additional Infomation |
Selcopintide acetate (Cpne7-DP acetate) is a research peptide for studying dentin regeneration and odontoblast differentiation. CPNE7 is a protein involved in tooth development and repair, and this synthetic peptide mimics its bioactive domain. The compound has not entered clinical trials or received regulatory approval for human use. It is supplied as an acetate salt for enhanced solubility and stability. Sequence: KYKQKRRSYK.
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| Molecular Formula |
C64H109N21O17
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| Related CAS # |
Selcopintide;2130912-34-2
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~100 mg/mL (~69.22 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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