| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
No specific biological target. Sulfo DBCO-PEG4-Maleimide TEA is a bioconjugation reagent. The DBCO group reacts with azides via copper-free click chemistry (SPAAC), while the maleimide group reacts specifically with sulfhydryl groups (thiols, typically from cysteine residues). The PEG4 spacer improves water solubility and reduces non-specific interactions.
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| ln Vitro |
Not applicable. Sulfo DBCO-PEG4-Maleimide TEA is a chemical linker used for the conjugation of biomolecules. It is not used as a modulator of biological activity but rather as a tool for generating labeled antibodies, proteins, or PROTAC degraders. The reactivity of both functional groups is maintained under mild, aqueous conditions.
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| ln Vivo |
Not applicable. Sulfo DBCO-PEG4-Maleimide TEA is not used as a therapeutic agent. It is used in the synthesis of PROTACs (proteolysis-targeting chimeras) and antibody-drug conjugates (ADCs). When incorporated into these molecules, it can contribute to in vivo efficacy by facilitating targeted protein degradation or tumor cell killing.
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| Enzyme Assay |
Non-cell-based conjugation assay: A thiol-containing protein (1 mg/mL in PBS, pH 7.4) is incubated with sulfo DBCO-PEG4-maleimide TEA (2-20 equivalents) for 2 h at room temperature. The maleimide-thiol reaction efficiency is assessed by mass spectrometry or SDS-PAGE. DBCO-azide click conjugation efficiency is assessed by incubating the maleimide-labeled protein with an azide-containing fluorophore for 1-4 h and measuring fluorescence.
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| Cell Assay |
Cell surface conjugation: Cells expressing azide-labeled surface glycans (via metabolic labeling with azido-sugars) are incubated with sulfo DBCO-PEG4-maleimide TEA (10-100 uM) for 30-60 min at 37degC. After washing, cells are fixed and analyzed by flow cytometry or confocal microscopy. The maleimide group can be used to attach thiol-functionalized cargoes.
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| Animal Protocol |
Not applicable as an independent drug. Sulfo DBCO-PEG4-Maleimide TEA is used for the ex vivo labeling of cells or for the preparation of conjugates that are subsequently administered in animal models. For example, a thiol-containing antibody is conjugated to an azide-containing payload via this reagent, and the resulting conjugate is injected into mice for pharmacokinetic or efficacy studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Sulfo DBCO-PEG4-Maleimide TEA alone are not relevant. As a PEG-containing reagent (MW 927.07), it is designed to improve the aqueous solubility and pharmacokinetic properties of the conjugates it creates. The PEG4 spacer reduces immunogenicity and increases circulating half-life when attached to proteins. The compound should be stored at -20degC, sealed, away from moisture.
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| Toxicity/Toxicokinetics |
Toxicity data for Sulfo DBCO-PEG4-Maleimide TEA are not reported, as it is a chemical linker for research use only. It should be handled with care to avoid inhalation, ingestion, and skin contact. Maleimide reagents can be skin irritants and may cause allergic reactions. The compound is not approved for human therapeutic or diagnostic use.
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| References |
[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562
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| Additional Infomation |
Sulfo DBCO-PEG4-Maleimide TEA is a research-grade click chemistry linker for bioconjugation, PROTAC synthesis, and ADC development. It is not a drug and has no therapeutic indications. The DBCO group enables copper-free click chemistry, which is desirable for live-cell and in vivo labeling. The TEA salt improves stability. For research use only.
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| Molecular Formula |
C45H62N6O13S
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|---|---|
| Related CAS # |
Sulfo DBCO-PEG4-Maleimide;2055198-07-5
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| Appearance |
Light yellow to yellow oil
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| SMILES |
O=C1C=CC(N1CCC(NCCOCCOCCOCCOCCC(NCC(S(=O)(O)=O)C(NCCC(N2C3=C(C#CC4=C(C2)C=CC=C4)C=CC=C3)=O)=O)=O)=O)=O.CCN(CC)CC
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~107.9 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.70 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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