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| Targets |
No specific biological target. Sulfo-Cy7 carboxylic acid TEA is a fluorescent labeling reagent for the covalent conjugation to biomolecules containing primary amines via carbodiimide (EDC) coupling. The NIR emission (773 nm) allows for deep tissue penetration and minimal background autofluorescence, making it suitable for in vivo imaging, flow cytometry, and immunoassays.
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| ln Vitro |
Not applicable. Sulfo-Cy7 carboxylic acid TEA is a fluorescent dye for biomolecular labeling and is not used as a modulator of biological activity. The TEA salt form ensures consistent labeling efficiency and stable solutions. The dye can be conjugated to antibodies, proteins, peptides, and nanoparticles for imaging applications.
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| ln Vivo |
Not applicable as a therapeutic agent. However, when conjugated to tumor-targeting antibodies or peptides, sulfo-Cy7 carboxylic acid TEA enables non-invasive near-infrared imaging of tumors in living animals. The dye provides high contrast and sensitivity for detecting small tumors due to its deep tissue penetration and low background.
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| Enzyme Assay |
Carboxylic acid activation and labeling (cell-free): Sulfo-Cy7 carboxylic acid TEA is dissolved in anhydrous DMSO (10 mg/mL). EDC (10-50 equivalents) and NHS (10-50 equivalents) are added and incubated for 15-30 min at room temperature to activate the carboxyl group. The activated dye is added to a protein solution (1-10 mg/mL in PBS, pH 7.4) and incubated for 2-4 h. Unreacted dye is removed by dialysis or size exclusion.
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| Cell Assay |
Cellular imaging protocol: Cells are incubated with a sulfo-Cy7-labeled targeting ligand (e.g., antibody, 1-10 ug/mL) for 30-60 min at 4degC or 37degC. After washing, cells are fixed and counterstained with DAPI. Images are acquired using a NIR fluorescence microscope or confocal laser scanning microscope (excitation 730-760 nm, emission 770-800 nm).
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| Animal Protocol |
NIR tumor imaging protocol: Tumor-bearing mice are injected intravenously with a sulfo-Cy7-labeled tumor-targeting antibody or peptide (1-10 mg of labeled conjugate per kg body weight). At 4-48 h post-injection, mice are anesthetized, and fluorescence images are acquired using an IVIS Spectrum or similar in vivo imaging system (excitation 745 nm, emission 800 nm). Signal-to-background ratios are calculated from regions of interest.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for sulfo-Cy7 carboxylic acid TEA alone are not reported, as it is a labeling reagent. The TEA salt form offers superior stability and a 3-year shelf life at -20degC compared to the free acid form. The dye is highly water-soluble due to its sulfonated structure, enabling direct aqueous conjugation without organic co-solvents.
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| Toxicity/Toxicokinetics |
Sulfo-Cy7 carboxylic acid TEA has low toxicity at imaging doses. No significant off-target effects or organ toxicity have been reported when used as a labeling reagent. The triethylammonium salt is not intended for human use. Standard safety precautions for handling dyes should be followed. The compound should be stored at -20degC in the dark.
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| References | |
| Additional Infomation |
Sulfo-Cy7 carboxylic acid TEA is a research-grade near-infrared dye for in vivo imaging, immunoassays, and protein labeling. It is not a drug and has no therapeutic applications. The TEA salt is preferred over the free acid for stability and consistency. CAS: 2104632-30-4; molecular weight: 770.01 (TEA salt). For research use only.
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| Molecular Formula |
C40H55N3O8S2
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| Molecular Weight |
770.01
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| Related CAS # |
Sulfo-Cy7 carboxylic acid;1251915-04-4
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| Appearance |
Light green to green solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~62.5 mg/mL (~81.17 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2987 mL | 6.4934 mL | 12.9868 mL | |
| 5 mM | 0.2597 mL | 1.2987 mL | 2.5974 mL | |
| 10 mM | 0.1299 mL | 0.6493 mL | 1.2987 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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