| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
No specific biological target. Sulfo-cyanine3 azide potassium is a fluorescent labeling reagent. It is used to covalently label alkyne-tagged biomolecules, including proteins, peptides, nucleic acids, and glycans, via click chemistry reactions. The sulfonate groups confer high water solubility and reduce dye-dye aggregation, making it suitable for use in fully aqueous biological systems.
|
|---|---|
| ln Vitro |
Not applicable. This compound is a fluorescent dye for biomolecular labeling and imaging. It is not used for modulating biological activity or for therapeutic applications. It is compatible with both copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry reactions.
|
| ln Vivo |
Not applicable. Sulfo-cyanine3 azide potassium is used for ex vivo labeling and imaging of biomolecules. It is suitable for in vivo imaging applications when conjugated to targeting moieties, providing bright and stable near-infrared fluorescence.
|
| Enzyme Assay |
Click chemistry conjugation (cell-free): An alkyne-modified biomolecule (0.1-1 mg/mL) is incubated with sulfo-cyanine3 azide potassium (2-10 equivalents) in PBS buffer (pH 7.4) containing CuSO4 (1 mM) and sodium ascorbate (5 mM) for 1 h at 37degC (CuAAC) or without copper for 2-4 h at room temperature (SPAAC). The labeled product is purified by size exclusion or dialysis, and labeling efficiency is determined by measuring absorbance at 548 nm.
|
| Cell Assay |
Cellular labeling protocol: Cells expressing alkyne-tagged biomolecules (e.g., metabolically labeled glycans or proteins) are fixed with 4% paraformaldehyde and permeabilized. Cells are incubated with sulfo-cyanine3 azide potassium (1-10 uM) in PBS containing CuSO4 (1 mM) and sodium ascorbate (5 mM) for 30-60 min at room temperature. After washing, cells are counterstained with DAPI and imaged by fluorescence microscopy.
|
| Animal Protocol |
In vivo imaging protocol: Mice bearing tumors or other tissues labeled with alkyne-tagged probes (e.g., via metabolic glycan labeling) are injected intravenously with sulfo-cyanine3 azide potassium (0.1-1 mg/kg). Following click chemistry reaction (using pre-targeting or in vivo click chemistry), fluorescence imaging is performed using an IVIS or similar imaging system (excitation 540-560 nm, emission 560-600 nm) at 0.5-4 h post-injection.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for sulfo-cyanine3 azide potassium alone are not relevant, as it is a fluorescent probe used for labeling. It has excellent aqueous solubility (0.62 M, ~456 g/L) due to sulfonate groups and a low aggregation tendency (absorption peak width 35-40 nm). The potassium salt form provides improved chemical stability compared to the free acid.
|
| Toxicity/Toxicokinetics |
Sulfo-cyanine3 azide potassium is not toxic at typical labeling concentrations (1-10 uM in vitro, 0.1-1 mg/kg in vivo). It is intended for research use only and not for human therapeutic or diagnostic applications. Standard safety precautions for handling dyes should be followed. The compound should be stored at -20degC, protected from light.
|
| References |
|
| Additional Infomation |
Sulfo-cyanine3 azide potassium is a research-grade fluorescent dye for biomolecular imaging and labeling. It is not a drug and has no therapeutic indications. The dye is compatible with live-cell labeling, immunofluorescence, and in vivo imaging studies. The molecular formula is C33H41KN6O7S2, and the molecular weight is 736.94. For research use only. Store at -20degC, protected from light.
|
| Molecular Formula |
C33H41KN6O7S2
|
|---|---|
| Molecular Weight |
736.94
|
| Related CAS # |
Sulfo-cyanine3 azide;1658416-54-6;Sulfo-cyanine3 azide sodium;2055138-89-9;Sulfo-cyanine3 azide TEA
|
| Appearance |
Brown to reddish brown solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3570 mL | 6.7848 mL | 13.5696 mL | |
| 5 mM | 0.2714 mL | 1.3570 mL | 2.7139 mL | |
| 10 mM | 0.1357 mL | 0.6785 mL | 1.3570 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
