| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| 1g |
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Purity: = 99.64%
| Targets |
Metabolic product of cystine
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|---|---|
| ln Vitro |
Thiotaurine, a metabolic product of cystine, contains a sulfane sulfur atom that can be released as H(2)S, a gaseous molecule with a regulatory activity on inflammatory responses. The influence of thiotaurine on human leukocyte spontaneous apoptosis has been evaluated by measuring caspase-3 activity in human neutrophils. Addition of 100 μM thiotaurine induced a 55% inhibition of caspase-3 activity similar to that exerted by 100 μM H(2)S. Interestingly, in the presence of 1 mM GSH, an increase of the inhibition of apoptosis by thiotaurine has been observed. These results indicate that the bioactivity of thiotaurine can be modulated by GSH, which promotes the reductive breakdown of the thiosulfonate generating H(2)S and hypotaurine. As thiotaurine is able to incorporate reversibly reduced sulfur, it is suggested that the biosynthesis of this thiosulfonate could be a means to transport and store H(2)S [1].
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| ln Vivo |
Thiotaurine is an effective antioxidant agent as demonstrated by its ability to counteract the damage caused by pro-oxidants in the rat [2].
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| Enzyme Assay |
Thiotaurine is a thiosulfonate compound bearing a sulfane sulfur atom metabolically generated in body fluids and tissues. Thiotaurine constitutes an interconnection molecule between aerobic and anaerobic pathways of cysteine metabolism. Thiotaurine formed as a result of the reaction between hypotaurine and sulfide may be converted back to H2S and hypotaurine. Thus, thiotaurine may be considered as a safe, non-toxic storage form of H2S and an important key intermediate in the biochemical routes of transport, storage and release of sulfide. Sulfane sulfur-containing compounds efficiently regulate the activity of enzymes and exhibit antioxidative properties. Interestingly, thiotaurine influences inflammatory processes modulating functional responses of human neutrophils and exhibits a protective effect against oxidative damage [2].
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| References |
[1]. Adv Exp Med Biol. 2013:775:227-36. doi: 10.1007/978-1-4614-6130-2_19.
[2]. Adv Exp Med Biol. 2019:1155:755-771. doi: 10.1007/978-981-13-8023-5_66. |
| Molecular Formula |
C2H7NO2S2
|
|---|---|
| Molecular Weight |
141.21248
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| Exact Mass |
140.992
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| Elemental Analysis |
Solid
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| CAS # |
2937-54-4
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| Appearance |
Solid
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| LogP |
0.89
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| tPSA |
93.35
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| SMILES |
NCCS(=S)(O)=O
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| InChi Key |
SHWIJIJNPFXOFS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C2H7NO2S2/c3-1-2-7(4,5)6/h1-3H2,(H,4,5,6)
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| Chemical Name |
2-hydroxysulfonothioylethanamine
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| Synonyms |
Thiotaurine; 2-hydroxysulfonothioylethanamine; 2-Aminoethanethiosulfonic acid; NQZ2D7AO62
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.0817 mL | 35.4083 mL | 70.8165 mL | |
| 5 mM | 1.4163 mL | 7.0817 mL | 14.1633 mL | |
| 10 mM | 0.7082 mL | 3.5408 mL | 7.0817 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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