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| Targets |
Nitric oxide synthase (NOS) and nitrogen metabolism pathways. L-Arginine is the physiological substrate for NOS, which catalyzes the production of nitric oxide (NO) and L-citrulline from L-arginine. The 15N-labeled form allows researchers to trace the fate of arginine-derived nitrogen in metabolic studies. L-Arginine-15N4 hydrochloride is also used in studies of cardiovascular health, immune function, and protein metabolism.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
L-Arginine-15N4 hydrochloride itself is not a pharmacologically active compound but rather a stable isotope-labeled tracer. The biological activity of the compound is identical to that of unlabeled L-arginine, as the isotopic substitution does not affect the compound's biochemical properties. L-Arginine is the precursor for NO synthesis via NOS and is also involved in protein synthesis and the urea cycle. |
| ln Vivo |
In vivo, L-Arginine-15N4 hydrochloride is used as a metabolic tracer to study nitrogen flux, protein turnover, and NO production. The 15N label allows researchers to track the incorporation of arginine-derived nitrogen into various metabolites and proteins. The compound has been used as a supplement for growing rat hybridoma to generate 15N-labeled monoclonal antibodies against GPIHBP1.
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| Enzyme Assay |
The in vitro enzyme/receptor binding (cell-free) assay for L-Arginine-15N4 hydrochloride is not a typical binding assay but rather a tracer-based assay. The compound is used as a substrate for NOS in enzymatic assays, where the production of 15N-labeled citrulline or NO can be monitored by mass spectrometry. The assay involves incubating NOS with L-Arginine-15N4 and cofactors, and measuring the isotopic enrichment of products.
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| Cell Assay |
In vitro cellular assays for L-Arginine-15N4 hydrochloride involve culturing cells in the presence of the labeled compound and measuring the incorporation of 15N into cellular metabolites, proteins, or NO. Cells are treated with L-Arginine-15N4, and the isotopic enrichment of intracellular arginine, citrulline, or proteins is analyzed by mass spectrometry. This allows researchers to study arginine metabolism, NOS activity, and protein synthesis rates.
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| Animal Protocol |
In vivo animal experiments for L-Arginine-15N4 hydrochloride involve administering the labeled compound to animals via oral gavage, intravenous injection, or in the diet. Blood, tissues, or urine samples are collected at various time points, and the isotopic enrichment of arginine, citrulline, and other metabolites is analyzed by mass spectrometry. This allows researchers to study whole-body arginine kinetics, NOS activity, and protein turnover.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for L-Arginine-15N4 hydrochloride are similar to those of unlabeled L-arginine. L-Arginine is absorbed from the gastrointestinal tract and distributed throughout the body. It is metabolized via multiple pathways, including NOS (producing NO and citrulline), arginase (producing urea and ornithine), and arginine:glycine amidinotransferase (producing creatine). The half-life of arginine in plasma is relatively short, approximately 1-2 hours.
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| Toxicity/Toxicokinetics |
As an isotopically labeled form of the endogenous amino acid L-arginine, L-Arginine-15N4 hydrochloride is not considered toxic at typical research doses. L-Arginine is a naturally occurring amino acid and is generally recognized as safe. The 15N isotope is stable and non-radioactive, posing no radiation hazard. No significant toxicities have been reported with the use of this compound in research settings.
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| References | |
| Additional Infomation |
L-Arginine-15N4 hydrochloride (CAS: 204633-95-4) has a molecular formula of C6H15ClN4O2 and a molecular weight of 214.64. The compound contains four 15N atoms, making it a valuable stable isotope tracer for studying arginine metabolism, protein synthesis, and nitric oxide production. It is widely used in research related to cardiovascular health, immune function, and protein metabolism. The compound is not approved for human use and is intended for research purposes only.
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| Molecular Formula |
C6H15CL15N4O2
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| Molecular Weight |
214.64
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| Exact Mass |
214.076
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| CAS # |
204633-95-4
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| Related CAS # |
L-Arginine hydrochloride;1119-34-2
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| PubChem CID |
16217518
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
13
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| Complexity |
176
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C(C[C@@H](C(=O)O)[15NH2])C[15N]=C([15NH2])[15NH2].Cl
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| InChi Key |
KWTQSFXGGICVPE-JYJSWXFRSA-N
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| InChi Code |
InChI=1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1/i7+1,8+1,9+1,10+1;
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| Chemical Name |
(2S)-2-(15N)azanyl-5-[bis(15N)(azanyl)methylideneamino]pentanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (1164.74 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6590 mL | 23.2948 mL | 46.5896 mL | |
| 5 mM | 0.9318 mL | 4.6590 mL | 9.3179 mL | |
| 10 mM | 0.4659 mL | 2.3295 mL | 4.6590 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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