| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 500mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
CaSR[1]
Calcium-sensing receptor (CaSR). Gadolinium chloride is a specific agonist of the calcium-sensing receptor (CaSR). By activating CaSR, it mimics the effects of extracellular calcium and modulates downstream signaling pathways involved in calcium homeostasis, cardiovascular function, and other physiological processes. |
|---|---|
| ln Vitro |
Gadolinium chloride (30 μM; 15 min; H9c2 cells) inhibits Bcl-2 expression in cells and increases Bax expression and caspase-3 activation in response to CsA [1]. GdCl3, a CaSR agonist, has been demonstrated to increase ERK1/2 phosphorylation and activate caspase-3 by inducing cardiomyocyte apoptosis in vitro [1].
Gadolinium chloride is a specific CaSR agonist. By activating the CaSR, it modulates calcium-sensing signaling pathways. Its activity has been demonstrated in various cellular models, where it induces CaSR-mediated responses similar to those elicited by extracellular calcium. It also decreased Bcl-2 expression in CsA-induced cells. |
| ln Vivo |
In vivo, Gadolinium chloride is used for the research of cardiovascular disease. By activating the CaSR, it can modulate vascular function and calcium homeostasis. Its effects in vivo would be assessed in animal models of cardiovascular disease and other CaSR-related disorders.
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| Enzyme Assay |
In vitro receptor binding and functional assays are used to characterize Gadolinium chloride as a CaSR agonist. Functional assays measuring CaSR-mediated signaling, such as intracellular calcium mobilization or MAPK pathway activation, are performed using CaSR-expressing cells to confirm its agonistic activity.
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: H9c2 cells Tested Concentrations: 30 μM Incubation Duration: 15 minutes Experimental Results: Resulted in increased Bax expression and caspase-3 activation, and diminished Bcl-2 expression in CsA-induced cells. Cellular assays for Gadolinium chloride involve treating CaSR-expressing cells with the compound and measuring receptor activation. Functional readouts include intracellular calcium mobilization, a key downstream signal following CaSR activation. These assays confirm the compound's agonistic activity at the CaSR. |
| Animal Protocol |
In vivo studies with Gadolinium chloride are performed in animal models of cardiovascular disease and other CaSR-related disorders. The compound is administered via injection, and its effects on vascular function, calcium homeostasis, and disease progression are assessed. Its role as a CaSR agonist makes it a valuable tool for studying this receptor in vivo.
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| ADME/Pharmacokinetics |
Gadolinium chloride has a molecular formula of Cl3Gd and a molecular weight of 263.61. It is a white inorganic halide hygroscopic solid that is soluble in water. As an inorganic salt, its pharmacokinetic properties would be characterized by rapid distribution and clearance. The compound should be stored under anhydrous conditions.
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| Toxicity/Toxicokinetics |
Gadolinium chloride is a specific CaSR agonist. As an inorganic salt, its toxicity profile would be related to its gadolinium content. Gadolinium-based compounds can have potential toxicity, including nephrotoxicity. Standard preclinical safety studies would be required to evaluate its safety for research applications.
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| References | |
| Additional Infomation |
Gadolinium trichloride is a gadolinium coordination compound that can act as a TRP channel blocker.
Gadolinium chloride (Gadolinium(III) chloride; GdCl3, CAS#: 10138-52-0) is a specific calcium-sensing receptor (CaSR) agonist. It is a white inorganic halide solid soluble in water. It is used for cardiovascular disease research and as a precursor for nanoparticle synthesis and imaging applications. It has a molecular formula of Cl3Gd and a molecular weight of 263.61. |
| Molecular Formula |
CL3GD
|
|---|---|
| Molecular Weight |
263.61
|
| Exact Mass |
262.831
|
| CAS # |
10138-52-0
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| PubChem CID |
61486
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| Appearance |
White to off-white solid powder
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| Density |
4.52
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| Boiling Point |
1580ºC
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| Melting Point |
609ºC
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| Vapour Pressure |
33900mmHg at 25°C
|
| LogP |
2.068
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
0
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| Rotatable Bond Count |
0
|
| Heavy Atom Count |
4
|
| Complexity |
8
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
Cl[Gd](Cl)Cl
|
| InChi Key |
MEANOSLIBWSCIT-UHFFFAOYSA-K
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| InChi Code |
InChI=1S/3ClH.Gd/h3*1H;/q;;;+3/p-3
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| Chemical Name |
trichlorogadolinium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
1M HCl: 100 mg/mL (379.35 mM)
DMSO: < 1 mg/mL H2O: < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7935 mL | 18.9674 mL | 37.9348 mL | |
| 5 mM | 0.7587 mL | 3.7935 mL | 7.5870 mL | |
| 10 mM | 0.3793 mL | 1.8967 mL | 3.7935 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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