| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| Other Sizes |
| Targets |
The primary targets of ICDH are its substrates, isocitrate and the cofactors NAD+ or NADP+, depending on the isoform. The enzyme is also regulated by allosteric effectors; for example, the NAD+-linked mitochondrial enzyme displays allosteric properties, while the NADP+-linked enzyme is non-allosteric.
|
|---|---|
| ln Vitro |
ICDH exhibits specific in vitro activity of 3-20 units/mg protein. One unit is defined as the amount of enzyme that will convert 1.0 μmole of isocitrate to α-ketoglutarate per minute at pH 7.4 and 37°C. The enzyme shows a high preference for NADP+, with only about 0.2% of the main activity obtained with NAD as the cofactor. It has been used in activity assays to study the effects of various compounds, such as thorium, on citric acid cycle enzymes, and as a standard to measure the impact of temperature on NADP-IDH activity.
|
| ln Vivo |
In vivo, ICDH participates in several critical physiological processes, including adaptation to hypoxia, histone demethylation, and DNA modification. Its activity is essential for maintaining cellular redox balance and providing intermediates for biosynthetic pathways.
|
| Enzyme Assay |
In vitro enzyme activity assays for ICDH typically involve measuring the reduction of NAD(P)+ to NAD(P)H spectrophotometrically at 340 nm. The assay mixture contains isocitrate, NADP+, magnesium chloride, and the enzyme sample in an appropriate buffer (e.g., Tris-HCl, pH 7.4). Activity is calculated based on the rate of absorbance increase. The detection range for such kits is typically 0.2 mmol/L to 20 mmol/L. Sample types for these assays include serum, plasma, tissue extracts, and cell lysates.
|
| Cell Assay |
Cellular ICDH activity is typically measured in cell lysates using colorimetric or spectrophotometric microplate assay kits. Cells are lysed in an appropriate buffer, and the lysates are incubated with isocitrate and NADP+ substrate. The production of NADPH is detected colorimetrically at OD 450 nm or fluorometrically. These kits are suitable for a variety of sample types, including serum, plasma, tissue extracts, cell lysates, and cell culture supernatants.
|
| Animal Protocol |
In vivo animal studies involving ICDH typically utilize rodent models to assess enzyme activity in various tissues following experimental interventions. Tissue homogenates are prepared from organs such as the liver, heart, or brain, and ICDH activity is measured using the same spectrophotometric methods as in vitro assays. Studies may investigate the effects of toxins, drugs, or genetic modifications on enzyme activity in specific tissues.
|
| ADME/Pharmacokinetics |
As an enzyme, ICDH does not have conventional pharmacokinetic properties like absorption, distribution, metabolism, and excretion (ADME). However, the enzyme is stable in buffered aqueous glycerol solutions and maintains activity under appropriate storage conditions (2-8°C). Its stability may be affected by temperature, pH, and the presence of cofactors or inhibitors. The compound is typically shipped on wet ice.
|
| Toxicity/Toxicokinetics |
ICDH is generally considered non-toxic as a research reagent. The enzyme itself is a naturally occurring protein and does not present significant toxicity concerns in laboratory settings. Standard laboratory safety practices should be followed when handling the enzyme preparation, including the use of personal protective equipment such as dust masks, eyeshields, and gloves.
|
| References | |
| Additional Infomation |
See other relationships...
ICDH (EC 1.1.1.42) is a widely used research tool in metabolism studies, and its activity is often measured as a marker of mitochondrial function and cellular metabolic status. It is involved in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. Mutations in the IDH1 and IDH2 genes are associated with certain cancers, making the enzyme a target of therapeutic interest. The product from porcine heart is a common source for research applications. |
| Molecular Formula |
C46H56O2
|
|---|---|
| Molecular Weight |
640.935653686523
|
| Exact Mass |
640.428
|
| CAS # |
9028-48-2
|
| PubChem CID |
77834905
|
| Appearance |
Colorless to light yellow liquid
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
48
|
| Complexity |
933
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
OC1CC(C)(C)C2C=CC(C(=CC3C=CC=CC=3)C)=CC=2C1(C)C.OC1CC(C)(C)C2C=C(C(=CC3C=CC=CC=3)C)C=CC=2C1(C)C
|
| InChi Key |
GQASXCYBZFOWQR-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/2C23H28O/c1-16(13-17-9-7-6-8-10-17)18-11-12-19-20(14-18)22(2,3)15-21(24)23(19,4)5;1-16(13-17-9-7-6-8-10-17)18-11-12-19-20(14-18)23(4,5)21(24)15-22(19,2)3/h2*6-14,21,24H,15H2,1-5H3
|
| Chemical Name |
1,1,4,4-tetramethyl-6-(1-phenylprop-1-en-2-yl)-2,3-dihydronaphthalen-2-ol;1,1,4,4-tetramethyl-7-(1-phenylprop-1-en-2-yl)-2,3-dihydronaphthalen-2-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5602 mL | 7.8010 mL | 15.6021 mL | |
| 5 mM | 0.3120 mL | 1.5602 mL | 3.1204 mL | |
| 10 mM | 0.1560 mL | 0.7801 mL | 1.5602 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
