| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
Not applicable; Stearic Acid-d35 is a stable isotope-labeled internal standard for analytical quantification, not a pharmacologically active drug targeting specific biological receptors. The unlabeled parent compound stearic acid is a long-chain dietary saturated fatty acid that serves as a major component of cocoa butter and is found in beef fat and vegetable oils. Unlike many long-chain saturated fatty acids, dietary stearic acid does not induce hypercholesterolemia or raise LDL-cholesterol. Stearic Acid-d35, however, is used solely as an internal standard for mass spectrometry and as a tracer in lipidomics studies.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Stearic Acid-d35 is intended for use as an internal standard and does not directly exert pharmacological effects in vitro. The unlabeled stearic acid is a long-chain saturated fatty acid that is a major component of cocoa butter and is found in beef fat and vegetable oils. In cell-free systems, Stearic Acid-d35 is used as a standard to calibrate GC-MS or LC-MS instruments for fatty acid analysis. It co-elutes with unlabeled stearic acid but is distinguished by its mass shift (M+35), allowing precise quantification by isotope dilution. It is not typically used for testing biological activity in cell-free enzyme assays. |
| ln Vivo |
Stearic Acid-d35 is not intended for in vivo use as a therapeutic agent but serves as an internal standard in pharmacokinetic and metabolism studies. It can be administered to animals as a tracer to study stearic acid absorption, distribution, metabolism, and excretion. Because it is fully deuterated (M+35), it can be distinguished from endogenous stearic acid by mass spectrometry, enabling accurate quantification of stearic acid flux in lipid metabolism studies. The compound is also used to investigate lipid metabolism, membrane dynamics, and fatty acid transport in biological systems. Stearic acid itself is a common dietary fatty acid that does not induce hypercholesterolemia.
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| Enzyme Assay |
For quantitative GC-MS analysis of stearic acid, prepare a stock solution of Stearic Acid-d35 in hexane or chloroform at 1 mg/mL. Add a known amount (e.g., 10-100 ng/mL) of the deuterated standard to biological samples (plasma, serum, tissue homogenates) after lipid extraction (e.g., Folch extraction or Bligh-Dyer method). Transesterify the extracted lipids to fatty acid methyl esters (FAMEs) by heating with methanolic HCl or boron trifluoride/methanol. Extract FAMEs with hexane. Separate on a GC column (e.g., DB-5ms or SP-2560) using a temperature gradient. Detect by electron ionization (EI) MS in selected ion monitoring (SIM) mode. Quantify by isotope dilution using the peak area ratio of stearic acid methyl ester to Stearic Acid-d35 methyl ester against a calibration curve.
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| Cell Assay |
For cell-based fatty acid metabolism studies, culture cells (e.g., hepatocytes, adipocytes, or macrophages) in fatty acid-free medium. Add Stearic Acid-d35 (conjugated to BSA at a ratio of 1:5 to 1:10) to the culture medium at concentrations ranging from 10-100 uM. Incubate for 1-24 hours. Harvest cells, wash with PBS to remove unbound fatty acids, and extract cellular lipids by the Folch method. Transesterify to FAMEs and analyze by GC-MS as described above. Use the deuterated standard to quantify stearic acid uptake, incorporation into different lipid classes (triglycerides, phospholipids, cholesterol esters), and beta-oxidation. This allows precise measurement of fatty acid flux without endogenous background interference.
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| Animal Protocol |
For in vivo lipid metabolism studies, administer Stearic Acid-d35 to animals (e.g., rats, mice) by oral gavage (e.g., 10-50 mg/kg in corn oil or olive oil) or intravenous injection (e.g., 5-10 mg/kg in lipid emulsion). Collect blood at multiple time points (0, 0.5, 1, 2, 4, 6, 8, 12, 24 h). Harvest tissues (liver, adipose, muscle, heart) at endpoint. Extract lipids from plasma and tissues, transesterify to FAMEs, and analyze by GC-MS as described above. Calculate pharmacokinetic parameters (Cmax, Tmax, AUC, half-life) and determine the incorporation of stearic acid into various lipid pools. The deuterated tracer allows precise quantification of fatty acid absorption and distribution without interference from endogenous stearic acid.
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| ADME/Pharmacokinetics |
Stearic Acid-d35: Molecular formula C1₈HD3₅O2 (or CD3(CD2)1₆COOH). Molecular weight: 319.69 g/mol (unlabeled stearic acid 284.48). Appearance: White to off-white crystalline solid. Purity: ≥99% deuterated forms (d1-d35). Solubility: DMF 30 mg/mL, DMSO 10 mg/mL, Ethanol 20 mg/mL. Also soluble in chloroform, hexane, and other organic solvents. Storage: Store powder at -20degC. Protect from light and moisture. Shipping: Room temperature. The compound is a fully deuterated internal standard for precision lipidomics and metabolic tracer studies.
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| Toxicity/Toxicokinetics |
Stearic Acid-d35 has low toxicity at typical analytical working concentrations (ng/mL to ug/mL). Stearic acid is a common dietary saturated fatty acid found in many foods and is generally recognized as safe (GRAS). The deuterated analog shares the same safety profile. At high pharmacological doses, stearic acid may cause gastrointestinal discomfort, but the compound is not intended for human consumption. Standard laboratory safety precautions should be followed when handling the pure compound: wear appropriate personal protective equipment (lab coat, gloves, safety glasses), avoid inhalation of powder and contact with skin/eyes, wash hands thoroughly after handling. Not for human therapeutic use. Always consult the Safety Data Sheet (SDS) for detailed safety information.
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| References | |
| Additional Infomation |
Stearic Acid-d35 (Octadecanoic-d35 acid) is a fully deuterated internal standard for the quantification of stearic acid by GC- or LC-MS. Stearic acid is a long-chain saturated fatty acid and a major component of cocoa butter, found in beef fat and vegetable oils. Unlike many long-chain saturated fatty acids, dietary stearic acid does not induce hypercholesterolemia or raise LDL-cholesterol. Stearic Acid-d35 is a fully deuterated form of stearic acid, in which 35 hydrogen atoms are replaced with deuterium, making it ideal for use in tracer studies and as an internal standard for lipidomics and quantitative mass spectrometry. It is also known as C18:0-d35, Octadecanoic Acid-d35, and FA 18:0-d35. The compound is supplied as a high-purity solid reference standard for research use only. Not for human use.
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| Molecular Formula |
C18HD35O2
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|---|---|
| Molecular Weight |
319.69
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| Exact Mass |
319.491
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| CAS # |
17660-51-4
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| Related CAS # |
Stearic acid;57-11-4
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| PubChem CID |
12346575
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| Appearance |
White to off-white solid powder
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| Density |
0.998g/cm3
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| Boiling Point |
361ºC(lit.)
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| Melting Point |
68-70ºC(lit.)
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| Flash Point |
162.4ºC
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| Vapour Pressure |
8.58E-06mmHg at 25°C
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| Index of Refraction |
1.455
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| LogP |
6.332
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
16
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| Heavy Atom Count |
20
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| Complexity |
202
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C([2H])([2H])C(=O)O
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| InChi Key |
QIQXTHQIDYTFRH-KNAXIHRDSA-N
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| InChi Code |
InChI=1S/C18H36O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h2-17H2,1H3,(H,19,20)/i1D3,2D2,3D2,4D2,5D2,6D2,7D2,8D2,9D2,10D2,11D2,12D2,13D2,14D2,15D2,16D2,17D2
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| Chemical Name |
2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,12,12,13,13,14,14,15,15,16,16,17,17,18,18,18-pentatriacontadeuteriooctadecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1280 mL | 15.6402 mL | 31.2803 mL | |
| 5 mM | 0.6256 mL | 3.1280 mL | 6.2561 mL | |
| 10 mM | 0.3128 mL | 1.5640 mL | 3.1280 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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