| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Indole-3-acetamide-d5 has no pharmacological target in mammalian systems as it is primarily a plant hormone intermediate. Its non-labeled parent compound is an intermediate in the biosynthesis of indole-3-acetic acid (auxin), a plant growth regulator that acts through TIR1/AFB auxin receptor proteins in plants. In plant biology, the indole-3-acetamide pathway represents an alternative auxin biosynthesis route.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope-labeled compound, Indole-3-acetamide-d5 is not tested for in vitro pharmacological activity in mammalian cells. In plant cell systems, it is used to trace the conversion of indole-3-acetamide to indole-3-acetic acid by amidohydrolase enzymes. The non-labeled indole-3-acetamide is a plant hormone intermediate with no defined activity in mammalian pharmacological assays. |
| ln Vivo |
Indole-3-acetamide-d5 has no in vivo activity in animals as it is a deuterium-labeled plant biochemical intermediate. In plant studies, it can be administered to plant tissues to trace auxin biosynthesis via the indole-3-acetamide pathway. The compound is used exclusively as an analytical standard and tracer and is not intended for therapeutic applications.
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| Enzyme Assay |
For in vitro enzyme assays using Indole-3-acetamide-d5 as a tracer, the compound is dissolved in an appropriate solvent such as DMSO or methanol to prepare a stock solution. The tracer is then added to plant enzyme extracts containing amidohydrolase activity. The reaction mixture (e.g., 100 microL total volume) is incubated at room temperature or 30degC for 0-60 minutes. The reaction is stopped by adding organic solvent, and products are analyzed by LC-MS to detect the conversion of labeled indole-3-acetamide to indole-3-acetic acid-d.
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| Cell Assay |
For cell-based studies, plant tissues (e.g., Arabidopsis seedlings) are incubated in medium containing Indole-3-acetamide-d5 at defined concentrations (e.g., 1-50 microM). After incubation periods (e.g., 0, 1, 2, 4, 8, 12, 24 hours), tissues are harvested and homogenized in extraction buffer (e.g., methanol containing 0.1% formic acid). The extracts are centrifuged and analyzed by LC-MS/MS to quantify the uptake of labeled compound and its conversion to indole-3-acetic acid-d.
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| Animal Protocol |
In vivo tracer studies are primarily conducted in plants, not in animals. For plant studies, Indole-3-acetamide-d5 is applied to plant tissues via root uptake from hydroponic medium or direct injection into stems/shoots. Plants are maintained under controlled growth conditions (light/dark cycle, temperature 22degC). After treatment periods of 0-24 hours, plant tissues are harvested, frozen in liquid nitrogen, and extracted for LC-MS/MS analysis to trace auxin biosynthesis.
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| ADME/Pharmacokinetics |
Indole-3-acetamide-d5 is an analytical standard and stable isotope tracer, not a therapeutic drug. No pharmacokinetic parameters have been established for this compound. When used in plant studies, uptake and metabolism follow the plant-specific pathways for indole-3-acetamide, with conversion to indole-3-acetic acid occurring via amidohydrolase activity. In mammalian systems, this compound is not expected to be systemically absorbed or metabolized to active products.
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| Toxicity/Toxicokinetics |
Indole-3-acetamide-d5 has low toxicity as it is used at microgram to milligram quantities for research purposes. The non-labeled indole-3-acetamide is a plant metabolite with no significant known toxicity in mammals. Standard laboratory safety precautions for handling organic compounds apply. The compound is not intended for human or veterinary consumption, and no formal toxicological studies have been conducted for this deuterium-labeled material.
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| References | |
| Additional Infomation |
Indole-3-acetamide-d5 is not a drug but a deuterium-labeled research reagent. It has no approved drug status, no clinical trial history, and is not intended for human use. The compound is used exclusively as an analytical standard and tracer in plant biology and biochemistry studies, particularly in research related to plant hormones (auxins). It is also employed as an internal standard for accurate quantification of indole-3-acetic acid and related metabolites by mass spectrometry. Available in high isotopic purity (99.99%).
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| Molecular Formula |
C10H5D5N2O
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|---|---|
| Molecular Weight |
179.23
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| Exact Mass |
174.079
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| CAS # |
1204700-53-7
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| Related CAS # |
Indole-3-acetamide;879-37-8
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| PubChem CID |
131668185
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
407.1±47.0 °C at 760 mmHg
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| Flash Point |
200.0±29.3 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.650
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| LogP |
0.79
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
13
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| Complexity |
205
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C1=C(C(=C2C(=C1[2H])C(=C(N2)[2H])CC(=O)N)[2H])[2H]
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| InChi Key |
ZOAMBXDOGPRZLP-SNOLXCFTSA-N
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| InChi Code |
InChI=1S/C10H10N2O/c11-10(13)5-7-6-12-9-4-2-1-3-8(7)9/h1-4,6,12H,5H2,(H2,11,13)/i1D,2D,3D,4D,6D
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| Chemical Name |
2-(2,4,5,6,7-pentadeuterio-1H-indol-3-yl)acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.5794 mL | 27.8971 mL | 55.7942 mL | |
| 5 mM | 1.1159 mL | 5.5794 mL | 11.1588 mL | |
| 10 mM | 0.5579 mL | 2.7897 mL | 5.5794 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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