| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Spermidine-d6 has no independent pharmacological target as a stable isotope internal standard. The unlabeled spermidine is a naturally occurring polyamine present in ribosomes and living tissues, involved in cellular growth, proliferation, and autophagy regulation. Spermidine maintains cell membrane stability, increases antioxidant enzyme activity, and improves photosystem II (PSII) expression. It also has functions in inflammation reduction, lipid metabolism regulation, and modulation of cell growth and death via the PI3K/Akt signaling pathway.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope internal standard, Spermidine-d6 is not tested for in vitro pharmacological activity. It is added to cell lysates, culture media, or biological extracts to enable accurate and precise quantification of endogenous spermidine by LC-MS/MS. The deuterated analog closely mimics the chemical and biological properties of native spermidine while being distinguishable by mass spectrometry, making it a valuable tool for studying polyamine metabolism, aging, and cellular processes. |
| ln Vivo |
Spermidine-d6 has no in vivo pharmacological activity as a therapeutic agent. It is used as an internal standard for quantifying spermidine in biological samples obtained from animal studies, including plasma, urine, and tissue homogenates. The deuterated version follows the same ADME properties as natural spermidine and is used to calibrate analytical methods without affecting physiological processes. Spermidine-d6 is particularly useful for studies on aging, cell biology, and polyamine metabolism.
|
| Enzyme Assay |
For in vitro LC-MS/MS quantification, Spermidine-d6 is dissolved in an appropriate solvent (e.g., water, methanol, or 0.1% formic acid) to prepare a stock solution (e.g., 1 mg/mL). The internal standard is added to biological samples (cell lysates, culture media, plasma, tissues) at a fixed concentration (e.g., 10-500 ng/mL). For tissue samples, homogenization in 0.1% formic acid in methanol or 5% trichloroacetic acid is performed. Proteins are precipitated by adding methanol or acetonitrile (3-5 volumes) followed by centrifugation (10,000-15,000 rpm, 10 minutes). The supernatant is analyzed by LC-MS/MS. For derivatization-based methods (GC-MS), dansyl chloride or benzoyl chloride derivatization is used. The analyte-to-internal standard peak area ratio is used for quantification, correcting for matrix effects and extraction recovery.
|
| Cell Assay |
For cell-based studies, cells (e.g., cancer cell lines, neuronal cells, or aging models) are cultured in standard medium (DMEM or RPMI-1640 with 10% FBS, 2 mM glutamine). After experimental treatments affecting polyamine metabolism (e.g., with DFMO, an ODC inhibitor; or with exogenous spermidine supplementation), cell pellets are collected and lysed. Spermidine-d6 is added to the lysates at a fixed concentration (e.g., 10-100 ng/mL). Following protein precipitation with methanol or acetonitrile containing 0.1% formic acid and centrifugation, the supernatant is analyzed by LC-MS/MS to quantify endogenous spermidine levels. Spermidine concentrations are normalized to protein content by BCA assay. For time-course studies, cells are incubated with Spermidine-d6 as a tracer to monitor polyamine turnover.
|
| Animal Protocol |
For in vivo studies, Spermidine-d6 is not typically administered to animals as a treatment. It is used as an internal standard for quantifying spermidine in biological samples obtained from animals in aging, metabolic, or pharmacological studies. After collection of plasma (by cardiac puncture or tail vein), urine, or tissue homogenates (liver, kidney, brain, muscle), the internal standard is added at a fixed concentration (e.g., 10-500 ng/mL). For tissue samples, homogenization in 5% trichloroacetic acid or 0.1% formic acid in methanol is performed. Following protein precipitation and centrifugation, samples are analyzed by LC-MS/MS or GC-MS to determine absolute spermidine concentrations. For tracer studies, Spermidine-d6 can be administered intraperitoneally or intravenously and its metabolic fate tracked by mass spectrometry.
|
| Toxicity/Toxicokinetics |
Toxicology data specific to Spermidine-d6 is not reported. The unlabeled spermidine is an endogenous polyamine present in all living cells, with toxic effects only at supraphysiological concentrations (millimolar range in vitro). At high doses, exogenous spermidine may cause gastrointestinal disturbances. The deuterated version is chemically identical except for isotopic substitution and exhibits the same safety profile. Standard laboratory safety precautions for handling polyamines (gloves, safety glasses, fume hood) are recommended. Not intended for human consumption.
|
| References | |
| Additional Infomation |
Spermidine-d6 is not a drug but a deuterium-labeled stable isotope internal standard. It has no approved therapeutic status, no clinical trial history as a therapeutic agent, and is not intended for human consumption. This compound is used for research applications including as an internal standard for LC-MS and GC-MS quantification of spermidine in plasma, tissues, and cell extracts (improving analytical accuracy and correcting for matrix effects), metabolomics studies for aging and cell biology research, and polyamine metabolism studies. Spermidine is a promising biomarker for colorectal cancer and other diseases. Available with ≥95% purity and ≥98 atom% D.
|
| Molecular Formula |
C7H19N3
|
|---|---|
| Molecular Weight |
151.282831430435
|
| Exact Mass |
151.195
|
| CAS # |
2514812-10-1
|
| Related CAS # |
Spermidine;124-20-9
|
| PubChem CID |
139024997
|
| Appearance |
Colorless to off-white liquid
|
| LogP |
-1
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
10
|
| Complexity |
56.8
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[2H]C([2H])(C([2H])([2H])N)C([2H])([2H])NCCCCN
|
| InChi Key |
ATHGHQPFGPMSJY-RCKJUGKUSA-N
|
| InChi Code |
InChI=1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2/i3D2,5D2,7D2
|
| Chemical Name |
N'-(3-amino-1,1,2,2,3,3-hexadeuteriopropyl)butane-1,4-diamine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6103 mL | 33.0513 mL | 66.1026 mL | |
| 5 mM | 1.3221 mL | 6.6103 mL | 13.2205 mL | |
| 10 mM | 0.6610 mL | 3.3051 mL | 6.6103 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
