| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Targets |
17a-Hydroxypregnenolone-d3 does not have a specific pharmacological target as it is a stable isotope-labeled tracer. 17a-Hydroxypregnenolone itself is a prohormone that forms dehydroepiandrosterone (DHEA). The labeled form is used to trace steroid hormone biosynthesis and metabolism. Its "target" in research is the steroidogenic pathway.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro activity of 17a-Hydroxypregnenolone-d3 is measured as its utility as a tracer in steroid hormone research. In cell culture and cell-free systems, the labeled prohormone is converted to downstream steroid hormones, allowing for tracking via mass spectrometry. Its "activity" is reflected in its metabolic conversion along the steroidogenic pathway. |
| ln Vivo |
In vivo, 17a-Hydroxypregnenolone-d3 is used to study steroid hormone biosynthesis and metabolism in living organisms. Administered orally or intravenously, the labeled prohormone is absorbed, distributed to steroidogenic tissues, and converted to DHEA and other steroids. These studies provide quantitative data on steroid hormone kinetics and regulation.
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| Enzyme Assay |
In vitro enzyme assays with 17a-Hydroxypregnenolone-d3 typically involve studying enzymes of steroidogenesis such as 17α-hydroxylase, 17,20-lyase, and 3β-hydroxysteroid dehydrogenase. The labeled substrate is incubated with enzyme preparations, and the reaction products are analyzed by mass spectrometry. These assays provide mechanistic insights into steroid hormone biosynthesis.
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| Cell Assay |
In vitro cell culture experiments with 17a-Hydroxypregnenolone-d3 involve treating steroidogenic cell lines (e.g., adrenal or gonadal cells) with the labeled prohormone. Cells are cultured to allow conversion to downstream steroids. Following incubation, cells and media are collected, and steroids are extracted for analysis by mass spectrometry to quantify steroidogenesis.
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| Animal Protocol |
In vivo animal experiments with 17a-Hydroxypregnenolone-d3 typically involve administering the labeled compound via injection to rodents. Blood, tissues (adrenal, gonads), and excreta are collected at various time points. Isotopic enrichment of 17a-hydroxypregnenolone and its metabolites (DHEA, androstenedione) is measured by mass spectrometry. These studies provide quantitative data on steroid hormone biosynthesis.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of 17a-Hydroxypregnenolone-d3 are essentially identical to those of natural 17a-hydroxypregnenolone. As a steroid hormone precursor, it is absorbed and distributed to steroidogenic tissues. It is metabolized through the steroidogenic pathway to DHEA and other steroids. The deuterium label allows for precise tracking of its metabolism.
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| Toxicity/Toxicokinetics |
17a-Hydroxypregnenolone-d3 has a low toxicity profile as a naturally occurring steroid hormone precursor. The deuterium label does not introduce additional toxicity. For research use, standard laboratory safety practices are sufficient. It is not considered a hazardous substance.
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| References | |
| Additional Infomation |
17a-Hydroxypregnenolone-d3 is a deuterium-labeled form of 17a-hydroxypregnenolone, a pregnane steroid and prohormone that forms dehydroepiandrosterone (DHEA). It is used as a tracer in steroid hormone research. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only.
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| Molecular Formula |
C21H29D3O3
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|---|---|
| Molecular Weight |
335.50
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| Exact Mass |
335.253
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| CAS # |
105078-92-0
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| Related CAS # |
17a-Hydroxypregnenolone;387-79-1
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| PubChem CID |
71434080
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
478.4±45.0 °C at 760 mmHg
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| Flash Point |
257.2±25.2 °C
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| Vapour Pressure |
0.0±2.7 mmHg at 25°C
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| Index of Refraction |
1.568
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| LogP |
3.38
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
24
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| Complexity |
596
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| Defined Atom Stereocenter Count |
7
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| SMILES |
[2H]C([2H])([2H])C(=O)[C@]1(CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C)O
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| InChi Key |
JERGUCIJOXJXHF-DBAXYKBZSA-N
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| InChi Code |
InChI=1S/C21H32O3/c1-13(22)21(24)11-8-18-16-5-4-14-12-15(23)6-9-19(14,2)17(16)7-10-20(18,21)3/h4,15-18,23-24H,5-12H2,1-3H3/t15-,16+,17-,18-,19-,20-,21-/m0/s1/i1D3
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| Chemical Name |
2,2,2-trideuterio-1-[(3S,8R,9S,10R,13S,14S,17R)-3,17-dihydroxy-10,13-dimethyl-1,2,3,4,7,8,9,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthren-17-yl]ethanone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9806 mL | 14.9031 mL | 29.8063 mL | |
| 5 mM | 0.5961 mL | 2.9806 mL | 5.9613 mL | |
| 10 mM | 0.2981 mL | 1.4903 mL | 2.9806 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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