| Size | Price | |
|---|---|---|
| Other Sizes |
Purity: ≥98%
| Targets |
5-Hydroxytryptamine creatinine sulfate monohydrate targets serotonin receptors in the brain and other tissues. Serotonin is involved in mood regulation, gastrointestinal function, and vascular tone. Its mechanism of action involves interaction with various serotonin receptor subtypes (5-HT1, 5-HT2, 5-HT3, etc.) and modulation of serotonergic signaling.
|
|---|---|
| ln Vitro |
In vitro, 5-Hydroxytryptamine creatinine sulfate monohydrate is used to study serotonin receptor binding and signaling. It is a stabilized form of serotonin that can be used in receptor binding assays, functional assays (e.g., cAMP accumulation, calcium mobilization), and cell-based studies of serotonergic neurotransmission. Its activity is measured by receptor activation and downstream signaling.
|
| ln Vivo |
In vivo, 5-Hydroxytryptamine creatinine sulfate monohydrate is used to study the physiological and pathological roles of serotonin. Serotonin is involved in mood regulation, gastrointestinal function, and vascular tone. The compound is used in research on depression, anxiety, migraine, and other disorders involving serotonergic dysfunction.
|
| Enzyme Assay |
In vitro receptor binding assays with 5-Hydroxytryptamine creatinine sulfate monohydrate involve incubating the compound with membranes expressing serotonin receptors (e.g., 5-HT1A, 5-HT2A) and measuring displacement of a radiolabeled ligand. Competition binding assays are performed to determine affinity (Ki) for different receptor subtypes. Functional assays measure receptor activation through second messenger production.
|
| Cell Assay |
In vitro cell culture experiments with 5-Hydroxytryptamine creatinine sulfate monohydrate involve treating neuronal cell lines or primary neurons with the compound to study serotonergic signaling. Cells are treated with various concentrations, and endpoints include assessment of receptor activation, calcium signaling, neuronal activity, and gene expression changes. These experiments characterize the cellular effects of serotonin.
|
| Animal Protocol |
In vivo animal experiments with 5-Hydroxytryptamine creatinine sulfate monohydrate typically involve administering the compound to animal models of depression, anxiety, or other neuropsychiatric disorders. The compound is administered via injection or infusion, and endpoints include assessment of behavioral changes (e.g., forced swim test, elevated plus maze), neurotransmitter levels, and receptor expression.
|
| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of 5-Hydroxytryptamine creatinine sulfate monohydrate reflect the behavior of serotonin. Serotonin is rapidly metabolized by monoamine oxidase (MAO) and does not cross the blood-brain barrier significantly. The creatinine sulfate salt form provides stability for research use. It has low oral bioavailability and is typically administered by injection in research settings.
|
| Toxicity/Toxicokinetics |
5-Hydroxytryptamine creatinine sulfate monohydrate has a moderate toxicity profile. Serotonin at high concentrations can cause serotonergic toxicity. It is handled as a hazardous chemical and appropriate safety precautions should be taken. Standard laboratory safety practices, including the use of gloves and eye protection, should be followed when handling this compound.
|
| Additional Infomation |
5-Hydroxytryptamine creatinine sulfate monohydrate is a stabilized salt form of serotonin used in research on serotonergic neurotransmission. It targets serotonin receptors and is involved in mood regulation, gastrointestinal function, and vascular tone. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only.
|
| Molecular Formula |
C14H23N5O7S
|
|---|---|
| Molecular Weight |
405.43
|
| Exact Mass |
405.131
|
| CAS # |
61-47-2
|
| Related CAS # |
5-Hydroxytryptamine-d4 creatinine sulfate monohydrate;2687960-56-9
|
| PubChem CID |
137244785
|
| Appearance |
White to off-white solid powder
|
| Boiling Point |
416.1ºC at 760 mmHg
|
| Melting Point |
~220 °C (dec.)
|
| Flash Point |
205.4ºC
|
| LogP |
1.787
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
27
|
| Complexity |
398
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CN1CC(=O)N=C1N.C1=CC2=C(C=C1O)C(=CN2)CCN.O.OS(=O)(=O)O
|
| InChi Key |
BKCXVJIGPVULPX-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H12N2O.C4H7N3O.H2O4S.H2O/c11-4-3-7-6-12-10-2-1-8(13)5-9(7)10;1-7-2-3(8)6-4(7)5;1-5(2,3)4;/h1-2,5-6,12-13H,3-4,11H2;2H2,1H3,(H2,5,6,8);(H2,1,2,3,4);1H2
|
| Chemical Name |
3-(2-aminoethyl)-1H-indol-5-ol;2-imino-1-methylimidazolidin-4-one;sulfuric acid;hydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
0.1 M HCL: 20 mg/mL (49.33 mM)
DMSO: 16.67 mg/mL (41.12 mM) |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4665 mL | 12.3326 mL | 24.6652 mL | |
| 5 mM | 0.4933 mL | 2.4665 mL | 4.9330 mL | |
| 10 mM | 0.2467 mL | 1.2333 mL | 2.4665 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA