| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
Zymosterol targets the sterol biosynthesis pathway, contributing to the production of membrane components and steroid hormones. As a cholesterol precursor, it is involved in the cholesterol biosynthetic pathway. Its "target" is the enzymatic machinery of cholesterol synthesis, including enzymes such as sterol reductases and methyltransferases. |
|---|---|
| ln Vitro |
Zymosterol is only metabolized to cholesterol in the crude endoplasmic reticulum of human fibroblasts [2].
In vitro, Zymosterol is used to study cholesterol biosynthesis and sterol metabolism. It serves as an intermediate in the sterol biosynthesis pathway. Its activity is studied in cell-free systems and cell cultures to investigate the regulation of cholesterol synthesis and the effects of inhibitors on sterol metabolism. |
| ln Vivo |
In vivo, Zymosterol plays a crucial role in the sterol biosynthesis pathway, contributing to the production of membrane components and steroid hormones. Its presence and function are essential for maintaining the structural integrity and fluidity of cell membranes. It is a key intermediate in the endogenous production of cholesterol.
|
| Enzyme Assay |
In vitro enzyme assays with Zymosterol typically involve studying enzymes of the cholesterol biosynthetic pathway, such as sterol reductase or sterol methyltransferase. The compound is used as a substrate or product in assays to characterize the activity of these enzymes. Standard assays involve incubating with enzyme preparations and analyzing products by GC-MS or LC-MS.
|
| Cell Assay |
In vitro cell culture experiments with Zymosterol involve treating cells (e.g., fibroblasts) to study cholesterol biosynthesis and sterol trafficking. Cells are treated with zymosterol or its precursors, and endpoints include assessment of sterol accumulation, subcellular localization, and effects on membrane properties. These experiments characterize the role of zymosterol in cellular sterol homeostasis.
|
| Animal Protocol |
In vivo animal experiments with Zymosterol are not commonly performed as the compound is an endogenous metabolite. If used, it would be in studies of cholesterol metabolism, where labeled zymosterol is administered to animals to trace sterol biosynthesis and distribution. These studies provide insights into cholesterol metabolism and related disorders.
|
| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of Zymosterol reflect its role as a metabolic intermediate. It is synthesized endogenously in the sterol biosynthesis pathway and is rapidly converted to cholesterol or other sterols. It is not administered as a drug, and its levels in tissues reflect the activity of the cholesterol biosynthetic pathway.
|
| Toxicity/Toxicokinetics |
Zymosterol has a low toxicity profile as it is a naturally occurring endogenous metabolite. It is not considered a hazardous substance. For research use, standard laboratory safety practices are sufficient. Comprehensive toxicology studies have not been published as the compound is a normal cellular metabolite.
|
| References |
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| Additional Infomation |
Zimosterol is a cholesterol alkyl group and a 3β-sterol. It is found in humans, Saccharomyces cerevisiae, and mice as a metabolite. It is derived from the hydrogenation of 5α-cholestane. Zimosterol has been reported in Homo sapiens, tomatoes, and other organisms with relevant data. Zimosterol is a metabolite found or produced in Saccharomyces cerevisiae.
Zymosterol is a biosynthetic metabolite of cholesterol and a precursor in the biosynthesis of cholesterol and other sterols. It plays a crucial role in the sterol biosynthesis pathway, contributing to the production of membrane components and steroid hormones. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only. |
| Molecular Formula |
C27H44O
|
|---|---|
| Molecular Weight |
384.64
|
| Exact Mass |
384.339
|
| CAS # |
128-33-6
|
| Related CAS # |
Zymosterol-d5;1246298-29-2
|
| PubChem CID |
92746
|
| Appearance |
White to off-white solid powder
|
| Density |
1g/cm3
|
| Boiling Point |
482.6ºC at 760mmHg
|
| Melting Point |
110 °C
|
| Flash Point |
209.1ºC
|
| Vapour Pressure |
2.45E-11mmHg at 25°C
|
| Index of Refraction |
1.535
|
| LogP |
7.452
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
28
|
| Complexity |
653
|
| Defined Atom Stereocenter Count |
7
|
| SMILES |
C[C@H](CCC=C(C)C)[C@H]1CC[C@@H]2[C@@]1(CCC3=C2CC[C@@H]4[C@@]3(CC[C@@H](C4)O)C)C
|
| InChi Key |
CGSJXLIKVBJVRY-XTGBIJOFSA-N
|
| InChi Code |
InChI=1S/C27H44O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h7,19-21,23-24,28H,6,8-17H2,1-5H3/t19-,20+,21+,23-,24+,26+,27-/m1/s1
|
| Chemical Name |
(3S,5S,10S,13R,14R,17R)-10,13-dimethyl-17-[(2R)-6-methylhept-5-en-2-yl]-2,3,4,5,6,7,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5998 mL | 12.9992 mL | 25.9983 mL | |
| 5 mM | 0.5200 mL | 2.5998 mL | 5.1997 mL | |
| 10 mM | 0.2600 mL | 1.2999 mL | 2.5998 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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