| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
3-Indoleacetic acid-2,2-d2 does not have a specific pharmacological target as it is a stable isotope-labeled tracer. 3-Indoleacetic acid itself is a plant auxin that regulates plant cell elongation and division. In research, the labeled form is used to study auxin metabolism, plant hormone activity, and signal transduction pathways. Its "target" in research is the metabolic pathways involving auxin in plants.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro activity of 3-Indoleacetic acid-2,2-d2 is measured as its utility as a tracer in plant biology studies. It can be added to cell culture media to induce plant cell elongation and division. Its "activity" is reflected in its metabolic incorporation and its ability to serve as a probe for studying auxin metabolism and signaling. The deuterium label allows for precise tracking using mass spectrometry. |
| ln Vivo |
In vivo, 3-Indoleacetic acid-2,2-d2 is used to study auxin metabolism, distribution, and signaling in living plants. Administered to plants, the labeled auxin is taken up, distributed to tissues, and metabolized. These studies provide quantitative data on auxin kinetics, transport, and the role of auxin in plant growth and development. It is a critical tool for plant hormone research.
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| Enzyme Assay |
In vitro enzyme assays with 3-Indoleacetic acid-2,2-d2 typically involve studying enzymes of auxin metabolism such as auxin oxidases, IAA-amino acid conjugating enzymes, and IAA decarboxylases. The labeled substrate is incubated with enzyme preparations, and the reaction products are analyzed by mass spectrometry. These assays provide mechanistic insights into enzyme kinetics and the regulation of auxin metabolism.
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| Cell Assay |
In vitro cell culture experiments with 3-Indoleacetic acid-2,2-d2 involve supplementing plant cell culture media with the labeled auxin. Plant cells are cultured to allow incorporation of the label into auxin metabolites. Following incubation, cells are harvested, and metabolites are extracted for analysis by mass spectrometry. These experiments are used to study auxin metabolism and signaling in plant cells.
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| Animal Protocol |
In vivo animal experiments with 3-Indoleacetic acid-2,2-d2 are not applicable as the compound is a plant hormone. It is used in plant studies, not animal models. In plant research, the compound is administered to plants via various methods (e.g., root application, foliar spray), and plant tissues are collected for analysis of auxin metabolites and signaling.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of 3-Indoleacetic acid-2,2-d2 are relevant to plant studies, not animal systems. In plants, auxin is transported polarly and metabolized through various pathways. The deuterium label allows for precise tracking of the compound's distribution and metabolism using mass spectrometry. Its behavior in plants reflects natural auxin dynamics.
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| Toxicity/Toxicokinetics |
3-Indoleacetic acid-2,2-d2 has a low toxicity profile as it is a stable isotope-labeled plant hormone. The deuterium label does not introduce additional toxicity. For research use, standard laboratory safety practices are sufficient. It is not considered a hazardous substance for plant research applications.
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| References | |
| Additional Infomation |
3-Indoleacetic acid-2,2-d2 is a deuterated form of the most common natural plant growth hormone, auxin. It is a critical tool for studies on plant hormone activity, auxin metabolism, and signal transduction pathways. The compound is used in plant biology research and is available for laboratory research use only. It is not a drug and has no therapeutic indications.
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| Molecular Formula |
C10H7D2NO2
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|---|---|
| Molecular Weight |
177.20
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| Exact Mass |
177.075
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| CAS # |
24420-86-8
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| Related CAS # |
3-Indoleacetic acid;87-51-4
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| PubChem CID |
16213582
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| Appearance |
Off-white to light brown solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
415.0±20.0 °C at 760 mmHg
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| Melting Point |
165-169ºC(lit.)
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| Flash Point |
204.8±21.8 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.694
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| LogP |
1.43
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
13
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| Complexity |
205
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])(C1=CNC2=CC=CC=C21)C(=O)O
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| InChi Key |
SEOVTRFCIGRIMH-BFWBPSQCSA-N
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| InChi Code |
InChI=1S/C10H9NO2/c12-10(13)5-7-6-11-9-4-2-1-3-8(7)9/h1-4,6,11H,5H2,(H,12,13)/i5D2
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| Chemical Name |
2,2-dideuterio-2-(1H-indol-3-yl)acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (141.08 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6433 mL | 28.2167 mL | 56.4334 mL | |
| 5 mM | 1.1287 mL | 5.6433 mL | 11.2867 mL | |
| 10 mM | 0.5643 mL | 2.8217 mL | 5.6433 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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