| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
N-Acetylputrescine targets polyamine metabolism and acetylation pathways. As a polyamine metabolite, it is involved in the regulation of cell growth and differentiation. It regulates cell proliferation and signal transduction mainly by participating in polyamine acetylation modification.
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|---|---|
| ln Vitro |
Human lymphocytes produce N-acetylputrescine when [14C]putrescine is present [4].
In vitro, N-Acetylputrescine is used to study polyamine metabolism, cell proliferation, and signal transduction. It regulates cell proliferation and signal transduction through polyamine acetylation modification. Its activity is measured by assessing cell proliferation, polyamine levels, and acetylation status in cultured cells. |
| ln Vivo |
In vivo, N-Acetylputrescine is an endogenous metabolite found in various biological systems. Serum levels are increased in patients with breast cancer. It can be used as a potential biomarker for the progression of squamous cell carcinoma of the lung (SCCL) and Parkinson's disease (PD).
|
| Enzyme Assay |
In vitro enzyme assays with N-Acetylputrescine typically involve studying diamine acetyltransferase and other enzymes involved in polyamine metabolism. The compound is used as a product or substrate in assays to characterize enzyme activity. Standard assays involve incubating with enzyme preparations and analyzing products by HPLC or LC-MS.
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| Cell Assay |
In vitro cell culture experiments with N-Acetylputrescine involve treating cells to study polyamine metabolism, cell proliferation, and signal transduction. Cells are treated with the compound, and endpoints include assessment of cell proliferation, polyamine levels, acetylation status, and gene expression changes. These experiments characterize the role of N-acetylputrescine in cellular function.
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| Animal Protocol |
In vivo animal experiments with N-Acetylputrescine typically involve measuring its levels in serum, tissues, or other biological samples as a biomarker. Animal models of cancer or neurodegenerative diseases are used to study changes in N-acetylputrescine levels and their correlation with disease progression.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of N-Acetylputrescine reflect its role as a metabolite. It is produced from putrescine by diamine acetyltransferase. Its levels in biological samples reflect polyamine metabolism. It may be increased in disease states such as breast cancer and Parkinson's disease.
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| Toxicity/Toxicokinetics |
N-Acetylputrescine has a low toxicity profile as a naturally occurring metabolite. For research use, standard laboratory safety practices are sufficient. It is not considered a hazardous substance. Comprehensive toxicology studies have not been published for this compound.
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| References |
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| Additional Infomation |
N-acetyl putrescine is an N-monoacetyl-α,ω-diamine, an N-monoacetyl derivative of putrescine. It is a metabolite, and also a mouse metabolite. It is an N-monoacetyl-α,ω-diamine, and also an N-substituted putrescine. It is the conjugate base of N-acetyl putrescine. N-acetyl putrescine is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). N-acetyl putrescine has also been reported in fruit flies, humans, and other organisms with relevant data. N-acetyl putrescine is a metabolite found or produced in Saccharomyces cerevisiae.
N-Acetylputrescine is a polyamine metabolite that regulates cell proliferation and signal transduction through polyamine acetylation modification. It is formed from putrescine by diamine acetyltransferase 1 in the brain. Serum levels are increased in breast cancer patients. It can be used as a biomarker for SCCL and Parkinson's disease. The compound is not a drug and has no therapeutic indications. |
| Molecular Formula |
C6H14N2O
|
|---|---|
| Molecular Weight |
130.19
|
| Exact Mass |
130.111
|
| CAS # |
5699-41-2
|
| PubChem CID |
122356
|
| Appearance |
Colorless to light yellow liquid
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| Density |
0.948g/cm3
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| Boiling Point |
301ºC at 760mmHg
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| Flash Point |
135.8ºC
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| Index of Refraction |
1.48
|
| LogP |
1.402
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
9
|
| Complexity |
83.1
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C(C([H])([H])[H])N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])N([H])[H]
|
| InChi Key |
KLZGKIDSEJWEDW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C6H14N2O/c1-6(9)8-5-3-2-4-7/h2-5,7H2,1H3,(H,8,9)
|
| Chemical Name |
N-(4-aminobutyl)acetamide
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 100 mg/mL (768.11 mM)
DMSO: 100 mg/mL (768.11 mM) |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.6811 mL | 38.4054 mL | 76.8108 mL | |
| 5 mM | 1.5362 mL | 7.6811 mL | 15.3622 mL | |
| 10 mM | 0.7681 mL | 3.8405 mL | 7.6811 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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