| Size | Price | Stock | Qty |
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| 5mg |
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| Targets |
Human Endogenous Metabolite
N1,N12-Diacetylspermine has no well-defined pharmacological target; its role is primarily as a biomarker. The unlabeled compound is a polyamine derivative involved in cellular catabolism. Its levels reflect the activity of polyamine metabolism, which is closely linked to cell proliferation. It is not a drug that binds to a specific receptor, but its upregulation is associated with the activation of oncogenic pathways in cancer cells. |
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| ln Vitro |
In vitro, N1,N12-Diacetylspermine is a marker of cellular proliferation and polyamine metabolism. It is produced in cells by the action of the enzyme spermidine/spermine N1-acetyltransferase (SSAT), a rate-limiting enzyme in polyamine catabolism. Researchers add this compound to cell cultures as an external standard to measure the levels of this endogenous metabolite by LC-MS, which can indicate the activation state of the polyamine pathway.
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| ln Vivo |
In vivo, N1,N12-Diacetylspermine is a natural metabolite found in blood and urine. Its concentration is typically very low in healthy individuals but can be significantly elevated in patients with various cancers, including lung, colorectal, and prostate cancers. It is not an exogenous therapeutic agent but is a promising, non-invasive diagnostic biomarker for the detection and monitoring of cancer recurrence.
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| Enzyme Assay |
For in vitro (non-cellular) experiments, N1,N12-Diacetylspermine dihydrochloride is used as an analytical standard. It is dissolved in a suitable solvent (e.g., water, methanol, or 0.1% formic acid) to prepare a stock solution. This standard is used to create calibration curves for LC-MS or GC-MS assays. It is also spiked into biological samples to serve as an internal standard or a quality control to ensure the accuracy of the measurement of the endogenous compound.
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| Cell Assay |
For in vitro cellular experiments, N1,N12-Diacetylspermine dihydrochloride is not typically a treatment but is used for analytical purposes. After treating cells with a drug or experimental condition, the cells are lysed. The compound, as a standard, is added to the lysate to help quantify the endogenous levels of N1,N12-Diacetylspermine. This measurement helps researchers assess the compound's potential as a biomarker of a specific cellular response, such as proliferation or apoptosis.
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| Animal Protocol |
For in vivo experiments, N1,N12-Diacetylspermine is not administered as a drug but is measured as an endogenous biomarker. In a rodent model of cancer, for example, urine or blood samples are collected. The stable isotope-labeled version of N1,N12-Diacetylspermine can be used as an internal standard to accurately quantify the host animal's baseline and treatment-altered levels of this metabolite, correlating its concentration with tumor burden or treatment efficacy.
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| ADME/Pharmacokinetics |
As an endogenous metabolite, N1,N12-Diacetylspermine is not a xenobiotic and has no defined pharmacokinetic (PK) profile as a drug. Its levels in the body are a result of polyamine synthesis and degradation. It is formed intracellularly and is then exported into the bloodstream for elimination. It is primarily cleared from the circulation by the kidneys and excreted in urine, where it is often measured.
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| Toxicity/Toxicokinetics |
N1,N12-Diacetylspermine is a naturally occurring endogenous metabolite and is not considered a toxic chemical at the concentrations normally found in the body. However, it is a key research target because its elevated levels are indicative of disease. The dihydrochloride salt may be an irritant. For research use, the amounts handled are typically low (milligrams), and standard laboratory safety practices, including the use of gloves and safety glasses, are sufficient.
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| Additional Infomation |
Diacetylspermine is a type of acetamide compound.
N1,N12-Diacetylspermine dihydrochloride is not an approved drug but a research-use biochemical. Its primary value is as a potential diagnostic and prognostic biomarker for cancer. It is used as a research standard in clinical studies to validate assays for detecting this compound in patient urine or blood samples. This research aims to develop non-invasive screening tests for early cancer detection, particularly for ovarian, lung, and colorectal cancers. |
| Molecular Formula |
C14H30N4O2.2CLH
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|---|---|
| Molecular Weight |
359.3355
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| Exact Mass |
358.19
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| CAS # |
77928-71-3
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| PubChem CID |
132679
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
13
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| Heavy Atom Count |
22
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| Complexity |
234
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)NCCCNCCCCNCCCNC(=O)C.Cl.Cl
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| InChi Key |
NQNXERHVLXYXRO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H30N4O2.2ClH/c1-13(19)17-11-5-9-15-7-3-4-8-16-10-6-12-18-14(2)20;;/h15-16H,3-12H2,1-2H3,(H,17,19)(H,18,20);2*1H
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| Chemical Name |
N-[3-[4-(3-acetamidopropylamino)butylamino]propyl]acetamide;dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7829 mL | 13.9144 mL | 27.8288 mL | |
| 5 mM | 0.5566 mL | 2.7829 mL | 5.5658 mL | |
| 10 mM | 0.2783 mL | 1.3914 mL | 2.7829 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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