| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Spermidine-d8 hydrochloride does not have a specific pharmacological target as it is a stable isotope-labeled tracer. Spermidine itself is a polyamine involved in cellular growth, differentiation, and stress responses. Spermidine hydrochloride maintains cell membrane stability, increases antioxidant enzyme activities, and improves photosystem II (PSII) and relevant gene expression.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, Spermidine-d8 hydrochloride is used as a tracer in polyamine metabolism studies. Spermidine hydrochloride maintains cell membrane stability, increases antioxidant enzyme activities, and significantly reduces H2O2 and O2.- content. Its "activity" is reflected in its metabolic incorporation and its ability to serve as a probe for studying polyamine biosynthesis and catabolism. |
| ln Vivo |
In vivo, Spermidine-d8 hydrochloride is used to study polyamine metabolism, distribution, and turnover in living organisms. Administered orally or intravenously, the labeled spermidine is absorbed, distributed to tissues, and incorporated into polyamine pools or metabolized. These studies provide quantitative data on polyamine kinetics and the role of polyamines in cell growth and differentiation.
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| Enzyme Assay |
In vitro enzyme assays with Spermidine-d8 hydrochloride typically involve studying enzymes of polyamine metabolism such as spermidine synthase, ornithine decarboxylase, and polyamine oxidases. The labeled substrate is incubated with enzyme preparations, and the reaction products are analyzed by mass spectrometry. These assays provide mechanistic insights into enzyme kinetics and polyamine metabolism.
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| Cell Assay |
In vitro cell culture experiments with Spermidine-d8 hydrochloride involve supplementing cell culture media with the labeled spermidine. Cells are cultured to allow incorporation of the label into cellular polyamine pools. Following incubation, cells are harvested, and polyamines are extracted for analysis by mass spectrometry. These experiments are used to study polyamine metabolism in various cell types.
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| Animal Protocol |
In vivo animal experiments with Spermidine-d8 hydrochloride typically involve administering the labeled compound via oral gavage or injection to rodents. Blood, tissues, and excreta are collected at various time points. Isotopic enrichment of spermidine and its metabolites is measured by mass spectrometry. These studies provide quantitative data on whole-body polyamine metabolism.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of Spermidine-d8 hydrochloride are essentially identical to those of natural spermidine. Spermidine is absorbed from the gastrointestinal tract, distributed throughout the body, and incorporated into polyamine pools or metabolized. The deuterium label allows for precise tracking of the compound's distribution and metabolism using mass spectrometry.
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| Toxicity/Toxicokinetics |
Spermidine-d8 hydrochloride has a low toxicity profile as spermidine is a naturally occurring polyamine. The deuterium label does not introduce additional toxicity. For research use, standard laboratory safety practices are sufficient. At normal physiological concentrations, spermidine is safe and well-tolerated.
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| References | |
| Additional Infomation |
Spermidine-d8 hydrochloride is a deuterium-labeled form of spermidine trihydrochloride used as a tracer in polyamine metabolism studies. Spermidine hydrochloride maintains cell membrane stability, increases antioxidant enzyme activities, and improves photosystem II (PSII) and gene expression. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only.
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| Molecular Formula |
C7H14D8CL3N3
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|---|---|
| Molecular Weight |
262.68
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| Exact Mass |
261.138
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| CAS # |
1173019-26-5
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| Related CAS # |
Spermidine hydrochloride;334-50-9
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| PubChem CID |
71310762
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| Appearance |
White to off-white solid powder
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| LogP |
3.861
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
13
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| Complexity |
56.8
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])(C([2H])([2H])C([2H])([2H])NCCCN)C([2H])([2H])N.Cl.Cl.Cl
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| InChi Key |
LCNBIHVSOPXFMR-BQVYHINQSA-N
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| InChi Code |
InChI=1S/C7H19N3.3ClH/c8-4-1-2-6-10-7-3-5-9;;;/h10H,1-9H2;3*1H/i1D2,2D2,4D2,6D2;;;
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| Chemical Name |
N'-(3-aminopropyl)-1,1,2,2,3,3,4,4-octadeuteriobutane-1,4-diamine;trihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8069 mL | 19.0346 mL | 38.0691 mL | |
| 5 mM | 0.7614 mL | 3.8069 mL | 7.6138 mL | |
| 10 mM | 0.3807 mL | 1.9035 mL | 3.8069 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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