| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Invertase targets the glycosidic bond in sucrose, catalyzing its hydrolysis to fructose and glucose. Its "target" is the sucrose substrate. It is used to produce invert sugar syrup from sugar beet or sugar cane. It can also prevent the crystallization of sugar in confectioneries and decrease sucrose content in fruit juices.
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| ln Vitro |
This substance is used to catalyze the hydrolysis of sucrose and is generated by deep microbial fermentation. Temperature range: optimum temperature is 45–55°C; effective temperature range is 20–60°C; pH range: optimum pH is 4.5–5.5; inhibitors: Fe3+, Cu2+, Hg+, Pb+
In vitro, Invertase activity is measured by its ability to hydrolyze sucrose to glucose and fructose. One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per minute at pH 4.5 at 55°C, or at pH 4.8 at 50°C. Its activity is studied in cell-free systems using purified enzyme or yeast extracts. |
| ln Vivo |
In vivo, Invertase is not administered as a therapeutic agent. It is an enzyme used in food processing and research applications. It is used in the confectionery industry for the production of invert syrup, to prevent sugar crystallization, and to decrease sucrose content in fruit juices.
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| Enzyme Assay |
In vitro enzyme assays for Invertase typically involve incubating the enzyme with sucrose in appropriate buffer at optimal pH (e.g., pH 4.5 or 4.8) and temperature (e.g., 50-55°C). The reaction is stopped by heating, and the glucose produced is measured by spectrophotometric methods using glucose oxidase or by chromatographic methods.
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| Cell Assay |
Invertase is not typically used in cell culture experiments as a compound to treat mammalian cells. However, it may be used in food science research or to modify carbohydrate content in culture media. Its primary applications are in the food industry and enzyme research.
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| Animal Protocol |
In vivo animal experiments with Invertase are not commonly performed as the enzyme is used in food processing and research applications rather than as a therapeutic agent. If used, it would be in studies of carbohydrate digestion or to modify dietary carbohydrates. Its primary significance is in food science and enzyme technology.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of Invertase are not applicable as it is an enzyme used in food processing, not a therapeutic agent. As a protein, it is not administered as a drug. Its activity and stability are characterized in vitro under controlled conditions. It is typically stored as a powder and used in food applications.
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| Toxicity/Toxicokinetics |
Invertase has a low toxicity profile. It is generally recognized as safe for use in food processing. It may cause mild irritation. Standard laboratory safety practices should be followed when handling. It is not classified as a highly hazardous substance. It is used to make pharmaceuticals and in food applications.
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| References | |
| Additional Infomation |
Invertase (EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose into fructose and glucose. It is usually obtained from yeast and is used in enzyme preparations and in the confectionery and pastry industries. It is used to produce invert syrup and prevent sugar crystallization. The enzyme is not a drug and has no therapeutic indications. It is available for research and food industry use.
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| Exact Mass |
259.904
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|---|---|
| CAS # |
9001-57-4
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| Appearance |
White to off-white solid powder
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| Density |
2.0±0.1 g/cm3
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| Boiling Point |
370.9±42.0 °C at 760 mmHg
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| Flash Point |
178.1±27.9 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.574
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| LogP |
0.38
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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