| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Vitamin K1-d7 does not have a specific pharmacological target as it is a stable isotope-labeled tracer. Vitamin K1 itself is essential for blood coagulation, bone metabolism, and vascular health. The deuterated form is used as a tracer in nutritional science and metabolic research to study vitamin K1 metabolism, absorption, and distribution.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro activity of Vitamin K1-d7 is measured as its utility as a tracer in metabolic studies. In cell culture and cell-free systems, the labeled vitamin K1 is used to study vitamin K metabolism, carboxylation reactions, and the activity of vitamin K-dependent proteins. Its "activity" is reflected in its metabolic incorporation and its ability to serve as a probe for studying vitamin K biology. |
| ln Vivo |
In vivo, Vitamin K1-d7 is used to study vitamin K1 metabolism, absorption, distribution, and bioavailability in living organisms. Administered orally or intravenously, the labeled vitamin K1 is absorbed, distributed to tissues (liver, bone, vasculature), and incorporated into metabolic pathways. These studies provide quantitative data on vitamin K homeostasis and its role in coagulation and bone metabolism.
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| Enzyme Assay |
In vitro enzyme assays with Vitamin K1-d7 typically involve using the compound as a tracer or internal standard in assays that measure vitamin K metabolism or related pathways. The deuterated standard is used to control for extraction efficiency and ionization suppression in mass spectrometry analysis. Standard protocols involve spiking samples with the deuterated standard and analyzing by LC-MS/MS.
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| Cell Assay |
Vitamin K1-d7 is not typically used in cell culture experiments as a bioactive compound for treating cells. However, it may be employed as a tracer in cell-based studies investigating vitamin K metabolism, carboxylation reactions, or the activity of vitamin K-dependent proteins. Cells such as hepatocytes or osteoblasts are cultured with the labeled compound, and metabolites are analyzed by mass spectrometry.
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| Animal Protocol |
In vivo animal experiments with Vitamin K1-d7 typically involve administering the labeled compound via oral gavage or injection to rodents. Blood, tissues (liver, bone), and excreta are collected at various time points. Isotopic enrichment of vitamin K1 and its metabolites is measured by mass spectrometry. These studies provide quantitative data on vitamin K1 absorption, distribution, metabolism, and excretion.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of Vitamin K1-d7 are essentially identical to those of natural vitamin K1. Vitamin K1 is absorbed from the gastrointestinal tract in the presence of dietary fats, transported in chylomicrons, and distributed to tissues. It is metabolized in the liver and excreted in bile and urine. The deuterium label allows for precise tracking of its distribution and metabolism using mass spectrometry.
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| Toxicity/Toxicokinetics |
Vitamin K1-d7 has a low toxicity profile since vitamin K1 is a natural vitamin with a wide safety margin. The deuterium label does not introduce additional toxicity. For research use, standard laboratory safety practices are sufficient. It is not considered a hazardous substance. It should be stored under appropriate conditions to maintain stability.
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| References |
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| Additional Infomation |
Vitamin K1-d7 is a deuterium-labeled form of vitamin K1 (phylloquinone), a natural vitamin essential for blood coagulation, bone, and vascular metabolism. It is used as a tracer in nutritional science and metabolic research to study vitamin K1 metabolism, absorption, and distribution. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only.
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| Molecular Formula |
C31H39D7O2
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|---|---|
| Molecular Weight |
457.74
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| Exact Mass |
457.394
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| CAS # |
1233937-39-7
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| Related CAS # |
Vitamin K1;84-80-0;cis-Vitamin K1;16033-41-3;Vitamin K1-d4;5172-18-9
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| PubChem CID |
56965123
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| Appearance |
Colorless to light yellow liquid
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| Density |
0.999 g/mL at 25°C
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| LogP |
9.157
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
33
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| Complexity |
696
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(CCC[C@H](CCC[C@H](CCC/C(=C/CC1C(=O)C2=C(C(=C(C([2H])=C2C(=O)C=1C([2H])([2H])[2H])[2H])[2H])[2H])/C)C)C)C
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| InChi Key |
MBWXNTAXLNYFJB-VKXGTQFMSA-N
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| InChi Code |
InChI=1S/C31H46O2/c1-22(2)12-9-13-23(3)14-10-15-24(4)16-11-17-25(5)20-21-27-26(6)30(32)28-18-7-8-19-29(28)31(27)33/h7-8,18-20,22-24H,9-17,21H2,1-6H3/b25-20+/i6D3,7D,8D,18D,19D
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| Chemical Name |
5,6,7,8-tetradeuterio-2-[(E)-3,7,11,15-tetramethylhexadec-2-enyl]-3-(trideuteriomethyl)naphthalene-1,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1846 mL | 10.9232 mL | 21.8465 mL | |
| 5 mM | 0.4369 mL | 2.1846 mL | 4.3693 mL | |
| 10 mM | 0.2185 mL | 1.0923 mL | 2.1846 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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