| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
L-Arginine-d7 has no pharmacological target as an internal standard. The unlabeled L-arginine is a semi-essential amino acid that acts as the primary nitrogen donor for the synthesis of nitric oxide (NO), a potent vasodilator and signaling molecule, via the enzyme nitric oxide synthase (NOS). It also serves as a precursor for the synthesis of agmatine, polyamines, and creatine, and activates the mTOR signaling pathway involved in cell growth.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As an internal standard, L-Arginine-d7 is not used to measure in vitro pharmacological activity. However, the unlabeled L-arginine is known to enhance protein synthesis, reduce blood pressure by promoting vasodilation, and modulate immune function by supporting T-cell proliferation and the killing activity of macrophages. It is a common and essential supplement in many cell culture media formulations for optimal cell growth. |
| ln Vivo |
In vivo, L-arginine is a critical amino acid for numerous physiological processes. It is the sole substrate for nitric oxide (NO) production, which is crucial for cardiovascular health (regulating blood pressure and preventing atherosclerosis). It also plays a key role in immune function, wound healing, and the secretion of growth hormone and insulin. The d7-labeled version is used to accurately quantify and track its metabolic fate in living organisms.
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| Enzyme Assay |
L-Arginine-d7 is used as an internal standard for in vitro analytical methods. It is typically dissolved in water or a 0.1% formic acid solution to prepare a stock solution. A fixed amount of this standard is then spiked into a biological sample (e.g., plasma, urine, cell lysate) before sample preparation. After steps like protein precipitation or solid-phase extraction, the sample is analyzed by LC-MS/MS. The signal ratio between unlabeled arginine and L-Arginine-d7 is used for precise quantification.
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| Cell Assay |
For in vitro cellular experiments, L-Arginine-d7 is not used as a treatment but as an analytical standard. It is added post-treatment to cell lysates or culture media at a fixed concentration prior to processing. Its primary role is to be an internal control for LC-MS analysis. This allows for the accurate measurement of changes in the concentration of endogenous unlabeled L-arginine and its metabolites, such as citrulline and ornithine, in response to a specific experimental treatment.
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| Animal Protocol |
For in vivo animal experiments, L-Arginine-d7 can be administered as a tracer to study arginine and nitric oxide (NO) metabolism. It is typically given to rodents via intravenous (IV) injection or oral gavage. Blood samples are collected at multiple time points, and the plasma is analyzed by LC-MS. The data collected allows researchers to calculate kinetic parameters like the rate of arginine appearance (Ra) and clearance (Cl) in the whole body.
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| ADME/Pharmacokinetics |
L-Arginine-d7 is a stable isotope tracer and has no unique PK parameters. The unlabeled L-arginine is an endogenously produced and diet-derived amino acid with a complex metabolism. It has a short half-life of about 60-90 minutes in plasma. It is cleared by multiple pathways, including conversion to nitric oxide (NO), ornithine, and agmatine. Its concentrations are tightly regulated by the liver and kidneys, which are the primary sites of its synthesis and catabolism.
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| Toxicity/Toxicokinetics |
L-Arginine-d7 is a stable isotope-labeled amino acid with low toxicity. The unlabeled L-arginine is a naturally occurring, conditionally essential amino acid that is generally recognized as safe (GRAS). At high doses, it is relatively well tolerated, though some individuals may experience mild gastrointestinal disturbances. The deuterium-labeled version is chemically identical and poses no additional toxic risk, especially when used in the small quantities required for analytical or tracer studies.
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| References | |
| Additional Infomation |
L-Arginine-d7 is not a drug but a research-use stable isotope-labeled internal standard. It is an essential tool for pharmaceutical research and clinical diagnostics, allowing for the accurate and precise measurement of L-arginine levels in complex biological matrices. It is critical for studying metabolic disorders involving the urea cycle, the role of arginine in cardiovascular disease via the NO pathway, and protein and amino acid metabolism in health and disease.
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| Molecular Formula |
C6H8D7CLN4O2
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|---|---|
| Molecular Weight |
217.71
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| Exact Mass |
217.132
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| CAS # |
204244-77-9
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| Related CAS # |
L-Arginine hydrochloride;1119-34-2
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| PubChem CID |
117065486
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| Appearance |
Off-white to light yellow solid powder
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
13
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| Complexity |
176
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H][C@@](C(=O)O)(C([2H])([2H])C([2H])([2H])C([2H])([2H])N=C(N)N)N.Cl
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| InChi Key |
KWTQSFXGGICVPE-AHQNDZJWSA-N
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| InChi Code |
InChI=1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1/i1D2,2D2,3D2,4D;
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| Chemical Name |
(2S)-2-amino-2,3,3,4,4,5,5-heptadeuterio-5-(diaminomethylideneamino)pentanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (1148.32 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5933 mL | 22.9663 mL | 45.9327 mL | |
| 5 mM | 0.9187 mL | 4.5933 mL | 9.1865 mL | |
| 10 mM | 0.4593 mL | 2.2966 mL | 4.5933 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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